The Vibrio genus is widespread in marine and brackish environments, and several species are human and animal pathogens of global importance. Vibrios adapt rapidly to many environmental stresses, so that brackish environments can be both a suitable niche and a possible reservoir for them. To test the occurrence of culturable vibrios and their possible correlation with environmental factors in a temperate brackish environment, a 1year sampling study was performed in three brackish ponds located along the Central Thyrrenian coast in the Macchiatonda Nature Reserve (Santa Marinella, district of Rome, Italy). Molecular methods were used to detect Vibrio cholerae, Vibrio arahaemolyticus, and Vibrio vulnificus pathogenicity associated genes among the Vibrio isolates. Out of 130 Vibrio isolates identified by sequencing a recA fragment, 70 harbored virulence associated genes including ctx, ace, tcpA, tdh, trh, vvhA, vllY, and toxRS, so confirming the spread of virulence determinants across the environmental isolates. Ecological analysis showed that, although the water temperature is known to be a strong predictor of abundance and distribution of vibrios, its influence accounts for 27 % of the observed variance in the Macchiatonda samples, increasing to 40 % when combined with salinity.

Matteucci, G., Schippa, S., DI LALLO, G., Migliore, L., Thaller, M.c. (2015). Species diversity, spatial distribution, and virulence associated genes of culturable vibrios in a brackish coastal Mediterranean environment. ANNALS OF MICROBIOLOGY, 65, 2311-2321 [10.1007/s13213-015-1073-6].

Species diversity, spatial distribution, and virulence associated genes of culturable vibrios in a brackish coastal Mediterranean environment

DI LALLO, GUSTAVO;MIGLIORE, LUCIANA;THALLER, MARIA CRISTINA
2015-03-20

Abstract

The Vibrio genus is widespread in marine and brackish environments, and several species are human and animal pathogens of global importance. Vibrios adapt rapidly to many environmental stresses, so that brackish environments can be both a suitable niche and a possible reservoir for them. To test the occurrence of culturable vibrios and their possible correlation with environmental factors in a temperate brackish environment, a 1year sampling study was performed in three brackish ponds located along the Central Thyrrenian coast in the Macchiatonda Nature Reserve (Santa Marinella, district of Rome, Italy). Molecular methods were used to detect Vibrio cholerae, Vibrio arahaemolyticus, and Vibrio vulnificus pathogenicity associated genes among the Vibrio isolates. Out of 130 Vibrio isolates identified by sequencing a recA fragment, 70 harbored virulence associated genes including ctx, ace, tcpA, tdh, trh, vvhA, vllY, and toxRS, so confirming the spread of virulence determinants across the environmental isolates. Ecological analysis showed that, although the water temperature is known to be a strong predictor of abundance and distribution of vibrios, its influence accounts for 27 % of the observed variance in the Macchiatonda samples, increasing to 40 % when combined with salinity.
20-mar-2015
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/19 - MICROBIOLOGIA GENERALE
Settore BIO/07 - ECOLOGIA
English
Con Impact Factor ISI
Vibrios, Brackish environment, Virulence associated genes
Pubblicazione online
Matteucci, G., Schippa, S., DI LALLO, G., Migliore, L., Thaller, M.c. (2015). Species diversity, spatial distribution, and virulence associated genes of culturable vibrios in a brackish coastal Mediterranean environment. ANNALS OF MICROBIOLOGY, 65, 2311-2321 [10.1007/s13213-015-1073-6].
Matteucci, G; Schippa, S; DI LALLO, G; Migliore, L; Thaller, Mc
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/115126
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