Honey has long been used in medicine for different purposes. Only recently, however, its antioxidant property and preventive effects against different diseases, such as cancer, have been highlighted. Chrysin (5,7-dihydroxyflavone) is a natural flavone commonly found in acacia honey. It has previously been shown to be an anti-tumor agent. In this study, we investigated the antiproliferative role of honey or chrysin on human (A375) and murine (B16-F1) melanoma cell lines. The results of the 3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide (MTT) assay and the trypan blue exclusion test showed that both the tested compounds were able to induce an antiproliferative effect on melanoma cells in a dose- and time-dependent manner. Flow cytometry analysis indicated that cytotoxicity induced by honey or chrysin was mediated by G0/G1 cell cycle arrest and induction of hyperploid progression. Our results suggest that the anti-proliferative effects of honey are due mainly to the presence of chrysin. Chrysin may therefore be considered a potential candidate for both cancer prevention and treatment. Further investigation is needed to validate the contribution of chrysin in tumor therapy in vivo. Introduction

Pichichero, E., Cicconi, R., Mattei, M., Gallinella Muzi, M., & Canini, A. (2010). Acacia honey and chrysin reduce proliferation of melanoma cells through aletrations in cell cycle progression. INTERNATIONAL JOURNAL OF ONCOLOGY, 37, 973-981 [10.3892/ijo_00000748].

Acacia honey and chrysin reduce proliferation of melanoma cells through aletrations in cell cycle progression

MATTEI, MAURIZIO;GALLINELLA MUZI, MARCO;CANINI, ANTONELLA
2010

Abstract

Honey has long been used in medicine for different purposes. Only recently, however, its antioxidant property and preventive effects against different diseases, such as cancer, have been highlighted. Chrysin (5,7-dihydroxyflavone) is a natural flavone commonly found in acacia honey. It has previously been shown to be an anti-tumor agent. In this study, we investigated the antiproliferative role of honey or chrysin on human (A375) and murine (B16-F1) melanoma cell lines. The results of the 3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide (MTT) assay and the trypan blue exclusion test showed that both the tested compounds were able to induce an antiproliferative effect on melanoma cells in a dose- and time-dependent manner. Flow cytometry analysis indicated that cytotoxicity induced by honey or chrysin was mediated by G0/G1 cell cycle arrest and induction of hyperploid progression. Our results suggest that the anti-proliferative effects of honey are due mainly to the presence of chrysin. Chrysin may therefore be considered a potential candidate for both cancer prevention and treatment. Further investigation is needed to validate the contribution of chrysin in tumor therapy in vivo. Introduction
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/01
English
Con Impact Factor ISI
acacia honey; chrysin; hyperploid cells; melanoma
Pichichero, E., Cicconi, R., Mattei, M., Gallinella Muzi, M., & Canini, A. (2010). Acacia honey and chrysin reduce proliferation of melanoma cells through aletrations in cell cycle progression. INTERNATIONAL JOURNAL OF ONCOLOGY, 37, 973-981 [10.3892/ijo_00000748].
Pichichero, E; Cicconi, R; Mattei, M; GALLINELLA MUZI, M; Canini, A
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/11504
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