This study was designed to evaluate the possibility of monitoring Mycobacterium tuberculosis infection using a serological assay. A discriminant score comprising antigen fractions of 38, 28, 24 and 19 kDa, identified in western blots using the Mycobacterium bovis bacille Calmette-Guérin (BCG) A60 antigen complex was established in a sample of 57 purified protein derivative (PPD)-negative and 47 PPD-positive individuals. It was then tested in a group of 140 subjects undergoing BCG vaccination as a model of tuberculosis complex infection and in a group of human immunodeficiency virus (HIV)-infected individuals as a model of cell-mediated immunodeficiency-related risk of tuberculosis. The discriminant score identified 57 out of 57 (100%) PPD-positives and none (0%) of the 47 PPD-negatives. In the BCG vaccinated subjects, 1.4% tested positive before vaccination and 90% after vaccination. In the HIV-positive subjects, 90% of the PPD-positive and 5% of the PPD-negative subjects had a positive score. This study suggests that the western blot discriminant score is an accurate test to survey M. tuberculosis infection in serum samples.

Rovatti, E., Corradi, M., Amicosante, M., Tartoni, P., Panini, W., Ancora, A., et al. (1996). Evaluation of a western blot serum test for the diagnosis of Mycobacterium tuberculosis infection. EUROPEAN RESPIRATORY JOURNAL, 9(2), 288-292.

Evaluation of a western blot serum test for the diagnosis of Mycobacterium tuberculosis infection

AMICOSANTE, MASSIMO;SALTINI, CESARE
1996-02-01

Abstract

This study was designed to evaluate the possibility of monitoring Mycobacterium tuberculosis infection using a serological assay. A discriminant score comprising antigen fractions of 38, 28, 24 and 19 kDa, identified in western blots using the Mycobacterium bovis bacille Calmette-Guérin (BCG) A60 antigen complex was established in a sample of 57 purified protein derivative (PPD)-negative and 47 PPD-positive individuals. It was then tested in a group of 140 subjects undergoing BCG vaccination as a model of tuberculosis complex infection and in a group of human immunodeficiency virus (HIV)-infected individuals as a model of cell-mediated immunodeficiency-related risk of tuberculosis. The discriminant score identified 57 out of 57 (100%) PPD-positives and none (0%) of the 47 PPD-negatives. In the BCG vaccinated subjects, 1.4% tested positive before vaccination and 90% after vaccination. In the HIV-positive subjects, 90% of the PPD-positive and 5% of the PPD-negative subjects had a positive score. This study suggests that the western blot discriminant score is an accurate test to survey M. tuberculosis infection in serum samples.
feb-1996
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/04 - PATOLOGIA GENERALE
English
BCG Vaccine; Membrane Glycoproteins; Humans; Mycobacterium bovis; Vaccination; Blotting, Western; HIV Seropositivity; HIV Infections; Serologic Tests; Antibodies, Bacterial; Adult; Antigens, Bacterial; Tuberculosis; Tuberculin Test; Female; Male; Mycobacterium tuberculosis
Rovatti, E., Corradi, M., Amicosante, M., Tartoni, P., Panini, W., Ancora, A., et al. (1996). Evaluation of a western blot serum test for the diagnosis of Mycobacterium tuberculosis infection. EUROPEAN RESPIRATORY JOURNAL, 9(2), 288-292.
Rovatti, E; Corradi, M; Amicosante, M; Tartoni, P; Panini, W; Ancora, A; Cenci, A; Zucchi, L; Monno, L; Angarano, G; Saltini, C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/107096
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