Berylliosis is a chronic granulomatous disorder caused by inhalation of Be dusts that is driven by the accumulation of Be-specific CD4+ Th1-cells at disease sites. Susceptibility to berylliosis has been associated with the supratypic variant of HLA-DP gene coding for glutamate at position beta69 (HLA-DPbetaGlu69). The aim of this study was to test the hypothesis that the HLA-DPbetaGlu69 residue plays a role in the interaction with Be. To this end, soluble HLA-DP2 molecule (carrying betaGlu69) and its mutated form carrying lysine at position beta69 (HLA-DP2Lys69) were produced in Drosophila melanogaster and then used in a Be binding assays. BeSO4 (1-1000 microM) was used to compete for the binding of the biotinilated invariant chain-derived peptide CLIP (50 microM). BeSO4 was capable of compete out biotin-CLIP binding from the HLA-DP2 (IC50%: 4.5 microM of BeSO4 at pH 5.0 and 5.5 microM of BeSO4 at pH 7.5), but not from the HLA-DP2Lys69 molecule (IC50%: 480 microM of BeSO4 at pH 5.0 and 220 microM of BeSO4 at pH 7.5). Moreover, the binding of NFLD.M60, a MoAb recognizing an epitope in the HLA-DP peptide binding region, to the HLA-DP2, but not to the HLA-DP2Lys69 soluble molecules was inhibited BeSO4. NFLD.M60 binding to HLA-DP2, but not to HLA-DP2Lys69 stably transfected murine cells was also inhibited by Be both at pH 5.0 and at pH 7.5. The data indicate a direct interaction of Be with the HLA-DPGlu69 molecule, in the absence of antigen processing.
Amicosante, M., Sanarico, N., Berretta, F., Arroyo, J., Lombardi, G., Lechler, R., et al. (2001). Beryllium binding to HLA-DP molecule carrying the marker of susceptibility to berylliosis glutamate beta 69. HUMAN IMMUNOLOGY, 62(7), 686-693.
|Tipologia:||Articolo su rivista|
|Citazione:||Amicosante, M., Sanarico, N., Berretta, F., Arroyo, J., Lombardi, G., Lechler, R., et al. (2001). Beryllium binding to HLA-DP molecule carrying the marker of susceptibility to berylliosis glutamate beta 69. HUMAN IMMUNOLOGY, 62(7), 686-693.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
Settore MED/10 - Malattie dell'Apparato Respiratorio
Settore MED/44 - Medicina del Lavoro
|Revisione (peer review):||Esperti anonimi|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||lug-2001|
|Titolo:||Beryllium binding to HLA-DP molecule carrying the marker of susceptibility to berylliosis glutamate beta 69|
|Autori:||Amicosante, M; Sanarico, N; Berretta, F; Arroyo, J; Lombardi, G; Lechler, R; Colizzi, V; Saltini, C|
|Appare nelle tipologie:||01 - Articolo su rivista|