In vitro diagnosis of MTB-infection uses MTB-proteins coded for by genes of the region of differentiation 1 (RD1) of the MTB genome. This study wants to test if proteins preferentially expressed during MTB-intracellular growth might provide new targets for the diagnosis of MTB-infection. To this end seventy-five multiepitopic HLA-promiscuous MTB-peptides were designed by quantitative implemented peptide-binding motif analysis from 3 MTB-protein genes expressed in activated human macrophages (MA), 4 genes expressed during growth in non-activated human macrophages (MN-A), 12 housekeeping genes (HKG) and 6 genes of the RD1 region (RD1) as control. ELISpot for IFN-was performed to measure the responses of PBMCs deriving from 45 patients affected by active tuberculosis and 34 controls. In active-TB patients, the mean response to RD1-derived peptides was higher than that to either MA (p<0.01), MN-A (p<0.008) or HKG (p<0.01) derived peptides. In TST-positive subjects all selected peptides elicited significant IFN-T-cell responses (p<0.02 compared to TST-negatives), but without differences between the subgroups. Further, T-cell responses to RD1 peptides were lower in the 23 active-TB treated patients than in the untreated ones (p<0.01). The response to MA peptides in treated active-TB was higher than when untreated (p<0.01). These results demonstrate that the use of in vitro models of MTB-intracellular infection to select MTB gene products for further in silico and in vitro assessment of their immunogenicity have the potential to identify novel antigens amenable to the design of new tools for diagnosis and monitoring of tuberculosis.

Seghrouchni, F., Contini, S., Markova, R., Drenska, R., Sadki, K., Baassi, L., et al. (2009). Design of immunogenic peptides from Mycobacterium tuberculosis genes expressed during macrophage infection. TUBERCULOSIS, 89(3), 210-217 [10.1016/j.tube.2009.03.005].

Design of immunogenic peptides from Mycobacterium tuberculosis genes expressed during macrophage infection

CONTINI, SILVIA;COLIZZI, VITTORIO;SALTINI, CESARE;AMICOSANTE, MASSIMO
2009-05-01

Abstract

In vitro diagnosis of MTB-infection uses MTB-proteins coded for by genes of the region of differentiation 1 (RD1) of the MTB genome. This study wants to test if proteins preferentially expressed during MTB-intracellular growth might provide new targets for the diagnosis of MTB-infection. To this end seventy-five multiepitopic HLA-promiscuous MTB-peptides were designed by quantitative implemented peptide-binding motif analysis from 3 MTB-protein genes expressed in activated human macrophages (MA), 4 genes expressed during growth in non-activated human macrophages (MN-A), 12 housekeeping genes (HKG) and 6 genes of the RD1 region (RD1) as control. ELISpot for IFN-was performed to measure the responses of PBMCs deriving from 45 patients affected by active tuberculosis and 34 controls. In active-TB patients, the mean response to RD1-derived peptides was higher than that to either MA (p<0.01), MN-A (p<0.008) or HKG (p<0.01) derived peptides. In TST-positive subjects all selected peptides elicited significant IFN-T-cell responses (p<0.02 compared to TST-negatives), but without differences between the subgroups. Further, T-cell responses to RD1 peptides were lower in the 23 active-TB treated patients than in the untreated ones (p<0.01). The response to MA peptides in treated active-TB was higher than when untreated (p<0.01). These results demonstrate that the use of in vitro models of MTB-intracellular infection to select MTB gene products for further in silico and in vitro assessment of their immunogenicity have the potential to identify novel antigens amenable to the design of new tools for diagnosis and monitoring of tuberculosis.
mag-2009
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/04 - PATOLOGIA GENERALE
Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
Settore MED/10 - MALATTIE DELL'APPARATO RESPIRATORIO
English
Macrophages; Interferon-gamma; Humans; Latent Tuberculosis; Sequence Analysis, Protein; Bacterial Proteins; Tuberculosis, Pulmonary; Adult; Antigens, Bacterial; Middle Aged; Peptides; Biological Markers; Female; Male; T-Lymphocytes; Mycobacterium tuberculosis
Seghrouchni, F., Contini, S., Markova, R., Drenska, R., Sadki, K., Baassi, L., et al. (2009). Design of immunogenic peptides from Mycobacterium tuberculosis genes expressed during macrophage infection. TUBERCULOSIS, 89(3), 210-217 [10.1016/j.tube.2009.03.005].
Seghrouchni, F; Contini, S; Markova, R; Drenska, R; Sadki, K; Baassi, L; Baassi, L; Todorova, Y; Terzieva, V; Bocchino, M; Cappelli, G; Altieri, A; Al...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/107050
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