In this study, we have investigated the neuroprotective actions of the membrane impermeable, lidocaine analog, N-ethyl lidocaine (QX-314) in the striatum. The effects of this drug were compared with those caused by the strictly-related-compound and sodium channel blocker lidocaine. To address this issue, electrophysiological recordings were performed in striatal slices, in control condition (normoxia) and during combined oxygen and glucose deprivation (in vitro ischemia). Either QX-314 or lidocaine induced, to some extent, a protection of the permanent electrophysiological alteration (field potential loss) caused by a period (12 min) of ischemia. Thus, both compounds permitted a partial recovery of the ischemic depression of the corticostriatal transmission and reduced the amplitude of the ischemic depolarization in medium spiny neurons. However, while QX-314, at the effective concentration of 100 microM, slightly reduced the amplitude of the excitatory field potential and did not affect the current-evoked spikes discharge of medium spiny striatal neurons, equimolar lidocaine depressed the field potential and eliminated repetitive spikes on a depolarizing step. On the basis of these observations, our results suggest the use of QX-314 as a neuroprotective agent in ischemic brain disorders.

Armogida, M., Giustizieri, M., Zona, C., Piccirilli, S., Nistico', R.g., Mercuri, N.b. (2010). N-ethyl lidocaine (QX-314) protects striatal neurons against ischemia: an in vitro electrophysiological study. SYNAPSE, 64(2), 161-168 [10.1002/syn.20735].

N-ethyl lidocaine (QX-314) protects striatal neurons against ischemia: an in vitro electrophysiological study

ZONA, CRISTINA;NISTICO', ROBERT GIOVANNI;MERCURI, NICOLA BIAGIO
2010-02-01

Abstract

In this study, we have investigated the neuroprotective actions of the membrane impermeable, lidocaine analog, N-ethyl lidocaine (QX-314) in the striatum. The effects of this drug were compared with those caused by the strictly-related-compound and sodium channel blocker lidocaine. To address this issue, electrophysiological recordings were performed in striatal slices, in control condition (normoxia) and during combined oxygen and glucose deprivation (in vitro ischemia). Either QX-314 or lidocaine induced, to some extent, a protection of the permanent electrophysiological alteration (field potential loss) caused by a period (12 min) of ischemia. Thus, both compounds permitted a partial recovery of the ischemic depression of the corticostriatal transmission and reduced the amplitude of the ischemic depolarization in medium spiny neurons. However, while QX-314, at the effective concentration of 100 microM, slightly reduced the amplitude of the excitatory field potential and did not affect the current-evoked spikes discharge of medium spiny striatal neurons, equimolar lidocaine depressed the field potential and eliminated repetitive spikes on a depolarizing step. On the basis of these observations, our results suggest the use of QX-314 as a neuroprotective agent in ischemic brain disorders.
feb-2010
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/09 - FISIOLOGIA
Settore MED/26 - NEUROLOGIA
English
Con Impact Factor ISI
glucose; male; sodium channel blockers; lidocaine; rats, wistar; rats; dose-response relationship, drug; neostriatum; animals; brain ischemia; sodium; microelectrodes; cerebral cortex; cell hypoxia; membrane potentials; neuroprotective agents; neurons; neural pathways
Armogida, M., Giustizieri, M., Zona, C., Piccirilli, S., Nistico', R.g., Mercuri, N.b. (2010). N-ethyl lidocaine (QX-314) protects striatal neurons against ischemia: an in vitro electrophysiological study. SYNAPSE, 64(2), 161-168 [10.1002/syn.20735].
Armogida, M; Giustizieri, M; Zona, C; Piccirilli, S; Nistico', Rg; Mercuri, Nb
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/10523
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