We characterized the expression and localization of pituitary adenylate cyclase-activating polypeptide (PACAP) and its specific type I receptor variants in prostatic, hyperplastic, and carcinomatous tissue collected from patients undergoing prostate biopsy and surgery for benign prostatic hyperplasia (BPH) and prostate cancer (PCa). The immunohistochemical studies using an indirect immunoperoxidase technique evidenced positive immunostaining for PACAP in the cytoplasm of epithelial cells of hyperplastic and carcinomatous prostate specimens and in some scattered cells of the stroma. Type I PACAP receptors (PAC1 R) in healthy and BPH tissues were localized in all epithelial cells lining the lumen of the acini and in some stromal cells, while in specimens from PCa the anti-PAC1 R antibody stained the apical portion of a large percentage of cells. Furthermore, our molecular studies provide evidence that several PAC1 R isoforms (null, SV1/SV2) are present in normal, hyperplastic, and neoplastic tissue, the null variant being the most intensely expressed in PCa. These observations provide additional evidence for a role of PACAP and PAC1 R in the events determining the outcome of PCa.

Moretti, C., Mammi, C., Frajese, G., Mariani, S., Gnessi, L., Arizzi, M., et al. (2006). PACAP and type I PACAP receptors in human prostate cancer tissue. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1070, 440-449 [10.1196/annals.1317.059].

PACAP and type I PACAP receptors in human prostate cancer tissue

MORETTI, COSTANZO;FRAJESE, GAETANO;
2006-07-01

Abstract

We characterized the expression and localization of pituitary adenylate cyclase-activating polypeptide (PACAP) and its specific type I receptor variants in prostatic, hyperplastic, and carcinomatous tissue collected from patients undergoing prostate biopsy and surgery for benign prostatic hyperplasia (BPH) and prostate cancer (PCa). The immunohistochemical studies using an indirect immunoperoxidase technique evidenced positive immunostaining for PACAP in the cytoplasm of epithelial cells of hyperplastic and carcinomatous prostate specimens and in some scattered cells of the stroma. Type I PACAP receptors (PAC1 R) in healthy and BPH tissues were localized in all epithelial cells lining the lumen of the acini and in some stromal cells, while in specimens from PCa the anti-PAC1 R antibody stained the apical portion of a large percentage of cells. Furthermore, our molecular studies provide evidence that several PAC1 R isoforms (null, SV1/SV2) are present in normal, hyperplastic, and neoplastic tissue, the null variant being the most intensely expressed in PCa. These observations provide additional evidence for a role of PACAP and PAC1 R in the events determining the outcome of PCa.
lug-2006
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/13 - ENDOCRINOLOGIA
English
Con Impact Factor ISI
Gene Expression Regulation, Neoplastic; Genetic Variation; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I; Humans; Aged; Middle Aged; Pituitary Adenylate Cyclase-Activating Polypeptide; Prostatic Neoplasms; Immunohistochemistry; Male
Moretti, C., Mammi, C., Frajese, G., Mariani, S., Gnessi, L., Arizzi, M., et al. (2006). PACAP and type I PACAP receptors in human prostate cancer tissue. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1070, 440-449 [10.1196/annals.1317.059].
Moretti, C; Mammi, C; Frajese, G; Mariani, S; Gnessi, L; Arizzi, M; Wannenes, F; Frajese, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/102992
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