Six Leptolyngbya strains, isolated from the archaeological surfaces of hypogean sites, were phenotypically and genetically characterized by light and electron microscopy and 16S rRNA gene and 16S-23S internally transcribed spacer (ITS) sequencing. Three phycoerythrin-rich (red) and three phycocyanin-rich (green) isolates were assigned to different operational taxonomic units (OTUs). Among the green isolates, one strain showed an OTU intraspecific variation due to differences in the ITS sequences and genomic polymorphism. Within the ITS sequence, variable regions, conserved domains and tRNA(Ile) and tRNA(Ala) genes showed high sequence identity among the phylotypes. Together, these data indicated a relatedness of the six strains to other Leptolyngbya from subaerophytic and geothermal environments and allowed the definition of novel Leptolyngbya OTUs.

Bruno, L., Billi, D., Bellezza, S., Albertano, P.b. (2009). Cytomorphological and genetic characterization of troglobitic Leptolyngbya strains isolated from roman hypogea. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(3), 608-617 [10.1128/AEM.01183-08].

Cytomorphological and genetic characterization of troglobitic Leptolyngbya strains isolated from roman hypogea

BRUNO, LAURA;BILLI, DANIELA;ALBERTANO, PATRIZIA BEATRICE
2009-01-01

Abstract

Six Leptolyngbya strains, isolated from the archaeological surfaces of hypogean sites, were phenotypically and genetically characterized by light and electron microscopy and 16S rRNA gene and 16S-23S internally transcribed spacer (ITS) sequencing. Three phycoerythrin-rich (red) and three phycocyanin-rich (green) isolates were assigned to different operational taxonomic units (OTUs). Among the green isolates, one strain showed an OTU intraspecific variation due to differences in the ITS sequences and genomic polymorphism. Within the ITS sequence, variable regions, conserved domains and tRNA(Ile) and tRNA(Ala) genes showed high sequence identity among the phylotypes. Together, these data indicated a relatedness of the six strains to other Leptolyngbya from subaerophytic and geothermal environments and allowed the definition of novel Leptolyngbya OTUs.
2009
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/01 - BOTANICA GENERALE
English
Bruno, L., Billi, D., Bellezza, S., Albertano, P.b. (2009). Cytomorphological and genetic characterization of troglobitic Leptolyngbya strains isolated from roman hypogea. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(3), 608-617 [10.1128/AEM.01183-08].
Bruno, L; Billi, D; Bellezza, S; Albertano, Pb
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/102694
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