HIV-1 preferentially infects CD4+ T cells, causing fundamental changes that eventually lead to the release of new viral particles and cell death. To investigate in detail alterations in the transcriptome of the CD4+ T cells upon viral infection, we sequenced polyadenylated RNA isolated from Jurkat cells infected or not with HIV-1. We found a marked global alteration of gene expression following infection, with an overall trend toward induction of genes, indicating widespread modification of the host biology. Annotation and pathway analysis of the most deregulated genes showed that viral infection produces a down-regulation of genes associated with the nucleolus, in particular those implicated in regulating the different steps of ribosome biogenesis, such as ribosomal RNA (rRNA) transcription, pre-rRNA processing, and ribosome maturation. The impact of HIV-1 infection on genes involved in ribosome biogenesis was further validated in primary CD4+ T cells. Moreover, we provided evidence by Northern Blot experiments, that host pre-rRNA processing in Jurkat cells might be perturbed during HIV-1 infection, thus strengthening the hypothesis of a crosstalk between nucleolar functions and viral pathogenesis.

Kleinman, C., Doria, M., Orecchini, E., Giuliani, E., Galardi, S., De Jay, N., et al. (2014). HIV-1 Infection Causes a Down-Regulation of Genes Involved in Ribosome Biogenesis. PLOS ONE [10.1371].

HIV-1 Infection Causes a Down-Regulation of Genes Involved in Ribosome Biogenesis

GALARDI, SILVIA;MICHIENZI, ALESSANDRO
2014-12-02

Abstract

HIV-1 preferentially infects CD4+ T cells, causing fundamental changes that eventually lead to the release of new viral particles and cell death. To investigate in detail alterations in the transcriptome of the CD4+ T cells upon viral infection, we sequenced polyadenylated RNA isolated from Jurkat cells infected or not with HIV-1. We found a marked global alteration of gene expression following infection, with an overall trend toward induction of genes, indicating widespread modification of the host biology. Annotation and pathway analysis of the most deregulated genes showed that viral infection produces a down-regulation of genes associated with the nucleolus, in particular those implicated in regulating the different steps of ribosome biogenesis, such as ribosomal RNA (rRNA) transcription, pre-rRNA processing, and ribosome maturation. The impact of HIV-1 infection on genes involved in ribosome biogenesis was further validated in primary CD4+ T cells. Moreover, we provided evidence by Northern Blot experiments, that host pre-rRNA processing in Jurkat cells might be perturbed during HIV-1 infection, thus strengthening the hypothesis of a crosstalk between nucleolar functions and viral pathogenesis.
2-dic-2014
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/13 - BIOLOGIA APPLICATA
English
Con Impact Factor ISI
Biosynthesis Gene expression HIV-1 Nucleolus RNA sequencing Ribosomes T cells Virus effects on host gene expression
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0113908
Kleinman, C., Doria, M., Orecchini, E., Giuliani, E., Galardi, S., De Jay, N., et al. (2014). HIV-1 Infection Causes a Down-Regulation of Genes Involved in Ribosome Biogenesis. PLOS ONE [10.1371].
Kleinman, C; Doria, M; Orecchini, E; Giuliani, E; Galardi, S; De Jay, N; Michienzi, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/101231
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