The early detection of genomic biomarkers (e.g. RNAs) through analysis of circulating blood cells could have a substantial impact on biomedicine, particularly in monitoring clinical trials, drug toxicity and doping in athletes. To achieve this goal, it is essential to develop methods that are sufficiently sensitive to detect biomarker alterations during normal biologic processes, pathogenic processes, and or in response to therapeutic or other intervention. Using a low density microarray (AndroChip 2) we detected a transcriptional profiling signature of 190 genes related to androgen and insulin metabolism pathway, in peripheral blood mononuclear cell (PBMC) in subjects with different intensities of sports activities. We demonstrated that androgen and insulin gene transcriptional levels are independent to sports activity and therefore potentially suitable for drug monitoring and/or drug doping (such as anabolic androgen steroid AAS abuse) and or gene doping.

Minella, D., Biancolella, M., Testa, B., Prosperini, G., Zenobi, R., Novelli, G., et al. (2010). Androgen- and insulin-related gene signature using a specific low density oligoarray androchip 2 in peripheral blood mononuclear cells in agonists, recreational athletes and sedentary subjects. JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS, 24(4), 413-423.

Androgen- and insulin-related gene signature using a specific low density oligoarray androchip 2 in peripheral blood mononuclear cells in agonists, recreational athletes and sedentary subjects.

BIANCOLELLA, MICHELA;ZENOBI, ROSSELLA;NOVELLI, GIUSEPPE;GIGANTI, MARIA GABRIELLA
2010-12-01

Abstract

The early detection of genomic biomarkers (e.g. RNAs) through analysis of circulating blood cells could have a substantial impact on biomedicine, particularly in monitoring clinical trials, drug toxicity and doping in athletes. To achieve this goal, it is essential to develop methods that are sufficiently sensitive to detect biomarker alterations during normal biologic processes, pathogenic processes, and or in response to therapeutic or other intervention. Using a low density microarray (AndroChip 2) we detected a transcriptional profiling signature of 190 genes related to androgen and insulin metabolism pathway, in peripheral blood mononuclear cell (PBMC) in subjects with different intensities of sports activities. We demonstrated that androgen and insulin gene transcriptional levels are independent to sports activity and therefore potentially suitable for drug monitoring and/or drug doping (such as anabolic androgen steroid AAS abuse) and or gene doping.
dic-2010
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/04 - PATOLOGIA GENERALE
English
Con Impact Factor ISI
anabolic androgen steroid; microarray; peripheral blood mononuclear cells
Minella, D., Biancolella, M., Testa, B., Prosperini, G., Zenobi, R., Novelli, G., et al. (2010). Androgen- and insulin-related gene signature using a specific low density oligoarray androchip 2 in peripheral blood mononuclear cells in agonists, recreational athletes and sedentary subjects. JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS, 24(4), 413-423.
Minella, D; Biancolella, M; Testa, B; Prosperini, G; Zenobi, R; Novelli, G; Giganti, Mg
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/9063
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