Fresnel zone plate based soft x-ray transmission microspectroscopy has developed into a routine technique for high-resolution elemental or chemical 2D imaging of thin film specimens. The availability of high resolution Fresnel lenses with short depth of focus offers the possibility of optical slicing (in the third dimension) by focus series with resolutions in the submicron regime. We introduce a 3D reconstruction algorithm that uses a variance-based metric to assign a focus measure as basis for volume rendering. The algorithm is applied to simulated geometries and opaque soft matter specimens thus enabling 3D visualization. These studies with z-resolution of few 100 nm serve as important step towards the vision of a confocal transmission x-ray microscope.

Spath, A., Scho, S., Riess, C., Schimidtel, D., Paradossi, G., Raabe, J., et al. (2014). STXM goes 3D: Digital reconstruction of focal stacks as novel approach towards confocal soft x-ray microscopy. ULTRAMICROSCOPY, 144, 19-25 [http://dx.doi.org/10.1016/j.ultramic.2014.04.004].

STXM goes 3D: Digital reconstruction of focal stacks as novel approach towards confocal soft x-ray microscopy

PARADOSSI, GAIO;
2014-01-01

Abstract

Fresnel zone plate based soft x-ray transmission microspectroscopy has developed into a routine technique for high-resolution elemental or chemical 2D imaging of thin film specimens. The availability of high resolution Fresnel lenses with short depth of focus offers the possibility of optical slicing (in the third dimension) by focus series with resolutions in the submicron regime. We introduce a 3D reconstruction algorithm that uses a variance-based metric to assign a focus measure as basis for volume rendering. The algorithm is applied to simulated geometries and opaque soft matter specimens thus enabling 3D visualization. These studies with z-resolution of few 100 nm serve as important step towards the vision of a confocal transmission x-ray microscope.
2014
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/02 - CHIMICA FISICA
English
X-ray microscopy; STXM; Confocal microscopy; Digital image reconstruction
Spath, A., Scho, S., Riess, C., Schimidtel, D., Paradossi, G., Raabe, J., et al. (2014). STXM goes 3D: Digital reconstruction of focal stacks as novel approach towards confocal soft x-ray microscopy. ULTRAMICROSCOPY, 144, 19-25 [http://dx.doi.org/10.1016/j.ultramic.2014.04.004].
Spath, A; Scho, S; Riess, C; Schimidtel, D; Paradossi, G; Raabe, J; Hornegger, J; Fink, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/84870
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