A large body of evidence shows that a single bout of strenuous exercise induces oxidative stress in circu- lating human lymphocytes leading to lipid peroxide- tion, DNA damage, mitochondrial perturbations, and protein oxidation. In a training experiment, Wistar rats were divided into control group (CG) and exer- cise group (EG). After a running level exercise until exhaustion, we observed an increase in the mRNA content and protein expression of SIRT1 and SIRT7 in the EG compared to the CG. Moreover, such train- ing exercise did not change mRNA transcripts and protein expression of FOXO3A and GADD45. We also observed an increase of pro-apoptotic protein bax and a decrease of the anti-apoptotic protein bcl-2 in the EG. Accordingly, we observed a caspase-3 activation and poly (ADP-ribose) polymerase (PARP) cleavage only in EG rats. Statistical analysis of the data showed a significant correlation between SIRT1 and SIRT7 expression and apoptotic proteins such as bax, bcl-2 in both tissues. We conclude that, in both muscle, such exercise activates both a damaging apoptotic mecha- nism with bax increase and bcl-2 decrease and a counterbalancing protective mechanism with SIRT1 and SIRT7 increase.

Marfe', G., Manzi, V., Tafani, M., Pucci, B., Gambacurta, A., Russo, M., et al. (2012). The modulation of sirtuins and apoptotic proteins in rats after exhaustive exercise. OPEN JOURNAL OF MOLECULAR AND INTEGRATIVE PHYSIOLOGY, 02(03), 65-74 [10.4236/ojmip.2012.23010].

The modulation of sirtuins and apoptotic proteins in rats after exhaustive exercise

MARFE', GABRIELLA;GAMBACURTA, ALESSANDRA;SINIBALDI SALIMEI, PAOLA
2012-01-01

Abstract

A large body of evidence shows that a single bout of strenuous exercise induces oxidative stress in circu- lating human lymphocytes leading to lipid peroxide- tion, DNA damage, mitochondrial perturbations, and protein oxidation. In a training experiment, Wistar rats were divided into control group (CG) and exer- cise group (EG). After a running level exercise until exhaustion, we observed an increase in the mRNA content and protein expression of SIRT1 and SIRT7 in the EG compared to the CG. Moreover, such train- ing exercise did not change mRNA transcripts and protein expression of FOXO3A and GADD45. We also observed an increase of pro-apoptotic protein bax and a decrease of the anti-apoptotic protein bcl-2 in the EG. Accordingly, we observed a caspase-3 activation and poly (ADP-ribose) polymerase (PARP) cleavage only in EG rats. Statistical analysis of the data showed a significant correlation between SIRT1 and SIRT7 expression and apoptotic proteins such as bax, bcl-2 in both tissues. We conclude that, in both muscle, such exercise activates both a damaging apoptotic mecha- nism with bax increase and bcl-2 decrease and a counterbalancing protective mechanism with SIRT1 and SIRT7 increase.
2012
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/05 - PATOLOGIA CLINICA
English
Con Impact Factor ISI
Marfe', G., Manzi, V., Tafani, M., Pucci, B., Gambacurta, A., Russo, M., et al. (2012). The modulation of sirtuins and apoptotic proteins in rats after exhaustive exercise. OPEN JOURNAL OF MOLECULAR AND INTEGRATIVE PHYSIOLOGY, 02(03), 65-74 [10.4236/ojmip.2012.23010].
Marfe', G; Manzi, V; Tafani, M; Pucci, B; Gambacurta, A; Russo, M; SINIBALDI SALIMEI, P
Articolo su rivista
File in questo prodotto:
File Dimensione Formato  
OJMIP20120300002_93394895.pdf

accesso aperto

Dimensione 1.71 MB
Formato Adobe PDF
1.71 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/82828
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact