Nuclear exclusion of the PTEN ( phosphatase and tensin homologue deleted in chromosome 10) tumour suppressor has been associated with cancer progression(1-6). However, the mechanisms leading to this aberrant PTEN localization in human cancers are currently unknown. We have previously reported that ubiquitinylation of PTEN at specific lysine residues regulates its nuclear cytoplasmic partitioning(7). Here we show that functional promyelocytic leukaemia protein ( PML) nuclear bodies co- ordinate PTEN localization by opposing the action of a previously unknown PTEN- deubiquitinylating enzyme, herpesvirus- associated ubiquitin- specific protease ( HAUSP, also known as USP7), and that the integrity of this molecular framework is required for PTEN to be able to enter the nucleus. We find that PTEN is aberrantly localized in acute promyelocytic leukaemia, in which PML function is disrupted by the PML - RAR alpha fusion oncoprotein. Remarkably, treatment with drugs that trigger PML - RAR alpha degradation, such as all- trans retinoic acid or arsenic trioxide, restore nuclear PTEN. We demonstrate that PML opposes the activity of HAUSP towards PTEN through a mechanism involving the adaptor protein DAXX ( death domain- associated protein). In support of this paradigm, we show that HAUSP is overexpressed in human prostate cancer and is associated with PTEN nuclear exclusion. Thus, our results delineate a previously unknown PML DAXX - HAUSP molecular network controlling PTEN deubiquitinylation and trafficking, which is perturbed by oncogenic cues in human cancer, in turn defining a new deubiquitinylation- dependent model for PTEN subcellular compartmentalization.

Song, M., Salmena, L., Carracedo, A., Egia, A., LO COCO, F., Teruya Feldstein, J., et al. (2008). The deubiquitinylation and localization of PTEN are regulated by a HAUSP-PML network. NATURE, 455(7214), 813-817 [10.1038/nature07290].

The deubiquitinylation and localization of PTEN are regulated by a HAUSP-PML network

LO COCO, FRANCESCO;
2008-01-01

Abstract

Nuclear exclusion of the PTEN ( phosphatase and tensin homologue deleted in chromosome 10) tumour suppressor has been associated with cancer progression(1-6). However, the mechanisms leading to this aberrant PTEN localization in human cancers are currently unknown. We have previously reported that ubiquitinylation of PTEN at specific lysine residues regulates its nuclear cytoplasmic partitioning(7). Here we show that functional promyelocytic leukaemia protein ( PML) nuclear bodies co- ordinate PTEN localization by opposing the action of a previously unknown PTEN- deubiquitinylating enzyme, herpesvirus- associated ubiquitin- specific protease ( HAUSP, also known as USP7), and that the integrity of this molecular framework is required for PTEN to be able to enter the nucleus. We find that PTEN is aberrantly localized in acute promyelocytic leukaemia, in which PML function is disrupted by the PML - RAR alpha fusion oncoprotein. Remarkably, treatment with drugs that trigger PML - RAR alpha degradation, such as all- trans retinoic acid or arsenic trioxide, restore nuclear PTEN. We demonstrate that PML opposes the activity of HAUSP towards PTEN through a mechanism involving the adaptor protein DAXX ( death domain- associated protein). In support of this paradigm, we show that HAUSP is overexpressed in human prostate cancer and is associated with PTEN nuclear exclusion. Thus, our results delineate a previously unknown PML DAXX - HAUSP molecular network controlling PTEN deubiquitinylation and trafficking, which is perturbed by oncogenic cues in human cancer, in turn defining a new deubiquitinylation- dependent model for PTEN subcellular compartmentalization.
2008
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/15 - MALATTIE DEL SANGUE
English
Con Impact Factor ISI
Daxx protein; enzyme; herpesvirus associated ubiquitin specific protease; phosphatase and tensin homologue deleted in chromosome 10; promyelocytic leukemia protein; tumor suppressor protein; unclassified drug; cancer; chromosome; cytoplasm; degradation; enzyme activity; protein; tumor; article; cancer growth; cell compartmentalization; cell culture; cell nucleus inclusion body; enzyme localization; human; human cell; human tissue; immunohistochemistry; priority journal; prostate cancer; protein synthesis; ubiquitination; active transport, cell nucleus; adaptor proteins, signal transducing;
Song, M., Salmena, L., Carracedo, A., Egia, A., LO COCO, F., Teruya Feldstein, J., et al. (2008). The deubiquitinylation and localization of PTEN are regulated by a HAUSP-PML network. NATURE, 455(7214), 813-817 [10.1038/nature07290].
Song, M; Salmena, L; Carracedo, A; Egia, A; LO COCO, F; Teruya Feldstein, J; Pandolfi, P
Articolo su rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/8120
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