Curcumin is a natural compound with recognized anti-inflammatory properties, but its anticancer activity is still object of study. We provided an unsupervised molecular investigation of the main proteome rearrangements involved in the cellular response to curcumin in a human neuroblastoma cell line sensitive to cisplatin and its resistant counterpart by a comparative proteomic approach. Shotgun analysis demonstrated that 66 proteins were differentially expressed in response to 24 h treatment with 40 μM curcumin in sensitive cells, whereas 32 proteins were significantly modulated in treated resistant cells. Functional analysis revealed that proteins involved in cellular assembly and organization, biosynthesis and glycolysis were down-regulated by curcumin treatment. Proteome changes were associated to cell cycle arrest in the G2/M phase and accumulation of polyubiquitinated proteins, also confirmed by flow cytometry and immunoblotting analysis, but not to a significant increment of reactive oxygen species production. Since the polyubiquitination of proteins influences a wide range of cellular pathways, the inhibition of the ubiquitin-proteasome system may be the main way through which curcumin performs its multi-target activity.

D'Aguanno, S., D'Agnano, I., De Canio, M., Rossi, C., Bernardini, S., Federici, G., et al. (2012). Shotgun proteomics and network analysis of neuroblastoma cell lines treated with curcumin. MOLECULAR BIOSYSTEMS, 8(4), 1068-1077 [10.1039/c2mb05498a].

Shotgun proteomics and network analysis of neuroblastoma cell lines treated with curcumin

D'AGUANNO, SIMONA;BERNARDINI, SERGIO;FEDERICI, GIORGIO;URBANI, ANDREA
2012-04-01

Abstract

Curcumin is a natural compound with recognized anti-inflammatory properties, but its anticancer activity is still object of study. We provided an unsupervised molecular investigation of the main proteome rearrangements involved in the cellular response to curcumin in a human neuroblastoma cell line sensitive to cisplatin and its resistant counterpart by a comparative proteomic approach. Shotgun analysis demonstrated that 66 proteins were differentially expressed in response to 24 h treatment with 40 μM curcumin in sensitive cells, whereas 32 proteins were significantly modulated in treated resistant cells. Functional analysis revealed that proteins involved in cellular assembly and organization, biosynthesis and glycolysis were down-regulated by curcumin treatment. Proteome changes were associated to cell cycle arrest in the G2/M phase and accumulation of polyubiquitinated proteins, also confirmed by flow cytometry and immunoblotting analysis, but not to a significant increment of reactive oxygen species production. Since the polyubiquitination of proteins influences a wide range of cellular pathways, the inhibition of the ubiquitin-proteasome system may be the main way through which curcumin performs its multi-target activity.
apr-2012
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA
English
Con Impact Factor ISI
Immunoblotting; Curcumin; Reproducibility of Results; Antineoplastic Agents; Dose-Response Relationship, Drug; Cell Cycle Checkpoints; Humans; Cell Line, Tumor; Drug Resistance, Neoplasm; Computational Biology; Reactive Oxygen Species; Neuroblastoma; Evaluation Studies as Topic; Cisplatin; Proteomics; Ubiquitination; Proteome; Flow Cytometry; G2 Phase; Cell Division
D'Aguanno, S., D'Agnano, I., De Canio, M., Rossi, C., Bernardini, S., Federici, G., et al. (2012). Shotgun proteomics and network analysis of neuroblastoma cell lines treated with curcumin. MOLECULAR BIOSYSTEMS, 8(4), 1068-1077 [10.1039/c2mb05498a].
D'Aguanno, S; D'Agnano, I; De Canio, M; Rossi, C; Bernardini, S; Federici, G; Urbani, A
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/77590
Citazioni
  • ???jsp.display-item.citation.pmc??? 10
  • Scopus 34
  • ???jsp.display-item.citation.isi??? 32
social impact