Cardiac progenitor cells (CPCs) are a promising autologous source of cells for cardiac regenerative medicine. However, CPC culture in vitro requires the presence of microenvironmental conditions (a complex array of bioactive substance concentration, mechanostructural factors, and physicochemical factors) closely mimicking the natural cell surrounding in vivo, including the capability to uphold reactive oxygen species (ROS) within physiological levels in vitro. Cerium oxide nanoparticles (nanoceria) are redox-active and could represent a potent tool to control the oxidative stress in isolated CPCs. Here, we report that 24 h exposure to 5, 10, and 50 !g/mL of nanoceria did not a!ect cell growth and function in cardiac progenitor cells, while being able to protect CPCs from H2O2-induced cytotoxicity for at least 7 days, indicating that nanoceria in an e!ective antioxidant. Therefore, these "ndings con"rm the great potential of nanoceria for controlling ROS-induced cell damage.

Pagliari, F., Mandoli, C., Forte, G., Magnani, E., Pagliari, S., Nardone, G., et al. (2012). Cerium Oxide Nanoparticles Protect Cardiac Progenitor Cells from Oxidative Stress. ACS NANO, 6, 3767-3775 [10.1021/nn2048069].

Cerium Oxide Nanoparticles Protect Cardiac Progenitor Cells from Oxidative Stress

LICOCCIA, SILVIA;MINIERI, MARILENA;DI NARDO, PAOLO;TRAVERSA, ENRICO
2012-01-01

Abstract

Cardiac progenitor cells (CPCs) are a promising autologous source of cells for cardiac regenerative medicine. However, CPC culture in vitro requires the presence of microenvironmental conditions (a complex array of bioactive substance concentration, mechanostructural factors, and physicochemical factors) closely mimicking the natural cell surrounding in vivo, including the capability to uphold reactive oxygen species (ROS) within physiological levels in vitro. Cerium oxide nanoparticles (nanoceria) are redox-active and could represent a potent tool to control the oxidative stress in isolated CPCs. Here, we report that 24 h exposure to 5, 10, and 50 !g/mL of nanoceria did not a!ect cell growth and function in cardiac progenitor cells, while being able to protect CPCs from H2O2-induced cytotoxicity for at least 7 days, indicating that nanoceria in an e!ective antioxidant. Therefore, these "ndings con"rm the great potential of nanoceria for controlling ROS-induced cell damage.
2012
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/07 - FONDAMENTI CHIMICI DELLE TECNOLOGIE
Settore ING-IND/22 - SCIENZA E TECNOLOGIA DEI MATERIALI
English
Con Impact Factor ISI
cerium oxide , nanoparticles . reactive oxygen species
Pagliari, F., Mandoli, C., Forte, G., Magnani, E., Pagliari, S., Nardone, G., et al. (2012). Cerium Oxide Nanoparticles Protect Cardiac Progenitor Cells from Oxidative Stress. ACS NANO, 6, 3767-3775 [10.1021/nn2048069].
Pagliari, F; Mandoli, C; Forte, G; Magnani, E; Pagliari, S; Nardone, G; Licoccia, S; Minieri, M; DI NARDO, P; Traversa, E
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/76208
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