An amperometric enzyme electrode for L-Alanine determination was developed. The probe is based on a platinum electrode which senses the hydrogen peroxide produced by the enzymatic reactions catalyzed by two enzymes: alanine aminotransferase (ALT) and glutamate oxidase. Both the enzymes were immobilized on polymeric membrane supports. Interferences were discriminated by additional protective membranes. Buffer, pH, cosubstrate, temperature and immobilization procedures were optimized. The probe response was linear in the concentration range - 10-3 mol/L with a detection limit of 2.10-6 mol/L. Studies of enzymatic interferences were carried out. Glutamine interferred about 50% at a concentration of 5-10-4 mol/L. Further studies were carried out to eliminate this interference. Analysis of alanine was performed in blood samples. Results compared with a HPLC procedure correlated well.
Palleschi, G., Volpe, G., D., C., M. G., L., MOSCONE DINIA, D., A., A. (1993). Amperometric Alanine Electrode. ANALYTICAL LETTERS, 26, 1301-1319.
Amperometric Alanine Electrode
PALLESCHI, GIUSEPPE;VOLPE, GIULIA;MOSCONE DINIA, DANILA;
1993-01-01
Abstract
An amperometric enzyme electrode for L-Alanine determination was developed. The probe is based on a platinum electrode which senses the hydrogen peroxide produced by the enzymatic reactions catalyzed by two enzymes: alanine aminotransferase (ALT) and glutamate oxidase. Both the enzymes were immobilized on polymeric membrane supports. Interferences were discriminated by additional protective membranes. Buffer, pH, cosubstrate, temperature and immobilization procedures were optimized. The probe response was linear in the concentration range - 10-3 mol/L with a detection limit of 2.10-6 mol/L. Studies of enzymatic interferences were carried out. Glutamine interferred about 50% at a concentration of 5-10-4 mol/L. Further studies were carried out to eliminate this interference. Analysis of alanine was performed in blood samples. Results compared with a HPLC procedure correlated well.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
