Rat neocortical neurons in cell culture were studied with the patch-clamp technique in order to determine the properties of a large-conductance K+ channel in excised inside-out patches. In the presence of a physiological ionic gradient for K+ across the patch membrane ([K+]i = 120 mM; [K+]o = 3 mM), outward channel activity was detected when the patches were brought to membrane potential values less negative than -30 mV. Depolarization of the membrane increased the magnitude of the current. The I-V relationship displayed rectification at negative membrane potentials. When the I-V curve was differentiated the slope conductance calculated at 0 mV membrane potential was 120 pS. The single-channel permeability was 5.2 x 10(-13) cm/s and the current flow through the open K+ channel could be modeled using the constant-field electrodiffusion theory. K+ channel opening was not observed following removal of Ca2+ from the intracellular surface of the membrane. Our experiments indicate that, as in other cell types, rat neocortical neurons in culture exhibit a large-conductance K+ channel which is activated by Ca2+ acting on the cytoplasmic surface.
Zona, C., & Avoli, M. (1989). Calcium-activated potassium channels recorded from rat neocortical neurons in cell culture. NEUROSCIENCE LETTERS, 102(2-3), 223-228.
|Tipologia:||Articolo su rivista|
|Citazione:||Zona, C., & Avoli, M. (1989). Calcium-activated potassium channels recorded from rat neocortical neurons in cell culture. NEUROSCIENCE LETTERS, 102(2-3), 223-228.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore BIO/09|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0304-3940(89)90082-7|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||31-lug-1989|
|Titolo:||Calcium-activated potassium channels recorded from rat neocortical neurons in cell culture|
|Autori:||Zona, C; Avoli, M|
|Appare nelle tipologie:||01 - Articolo su rivista|