Purified preparations of FC receptors from maize, obtained under non-denaturing conditions, showed in SDS-PAGE two doublets of proteins with an apparent molecular mass of 30 and 90 kDa. In this paper the isolation of the 30 kDa protein, its identification as a 14-3-3-like protein, as well as its immunological detection in partially-purified FC-receptor preparations from bean and spinach are described. The 14-3-3 proteins have biochemical properties consistent with potential signalling roles, and their presence in highly purified FC-receptor preparations suggests that they may be involved in FC- signal transduction. Photoaffinity labelling experiments demonstrating that the protein at 90 kDa binds FC are also presented. The evidence presented taken as a whole, suggests the occurrence in maize of a protein complex for FC perception and signal transduction.

Aducci, P., Ballio, A., Nasta, D., Fogliano, V., Fullone, M., Marra, M. (1996). Fusicoccin and its receptors - Perception and signal transduction. In Plant growth regulation (pp.93-98). Dordrecht : Kluwer.

Fusicoccin and its receptors - Perception and signal transduction

ADUCCI, PATRIZIA;MARRA, MAURO
1996-01-01

Abstract

Purified preparations of FC receptors from maize, obtained under non-denaturing conditions, showed in SDS-PAGE two doublets of proteins with an apparent molecular mass of 30 and 90 kDa. In this paper the isolation of the 30 kDa protein, its identification as a 14-3-3-like protein, as well as its immunological detection in partially-purified FC-receptor preparations from bean and spinach are described. The 14-3-3 proteins have biochemical properties consistent with potential signalling roles, and their presence in highly purified FC-receptor preparations suggests that they may be involved in FC- signal transduction. Photoaffinity labelling experiments demonstrating that the protein at 90 kDa binds FC are also presented. The evidence presented taken as a whole, suggests the occurrence in maize of a protein complex for FC perception and signal transduction.
International symposium on plant hormone signal perception and transduction
Moscow
1994
Rilevanza internazionale
1996
Settore BIO/04 - FISIOLOGIA VEGETALE
English
fusicoccin; receptors; signal transduction; 14-3-3 protein; photoaffinity labelling
Intervento a convegno
Aducci, P., Ballio, A., Nasta, D., Fogliano, V., Fullone, M., Marra, M. (1996). Fusicoccin and its receptors - Perception and signal transduction. In Plant growth regulation (pp.93-98). Dordrecht : Kluwer.
Aducci, P; Ballio, A; Nasta, D; Fogliano, V; Fullone, M; Marra, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/6120
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