Tryptophan 83, a residue strongly involved in the intersubunit interaction of the Cu,Zn superoxide dismutases from Photobacterium leiognathi, has been selectively mutated to phenylalanine or tyrosine. The recombinant mutant enzymes expressed in Escherichia coil were purified in two well distinct and stable forms, one dimeric and fully active and the other monomeric and devoid of metals. In agreement, in vitro experiments indicate that the removal and addition of zinc in the mutant enzymes induces monomerization and dimerization, respectively, while does not perturb the dimeric association of the native protein. This is the first unambiguous experimental proof of a direct communication between the intersubunit interface and the metal active site. (C) 2000 Academic Press.

D'Orazio, M., Battistoni, A., Stroppolo, M., Desideri, A. (2000). Single mutation induces a metal-dependent subunit association in dimeric Cu,Zn superoxide dismutase. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 272(1), 81-83 [10.1006/bbrc.2000.2730].

Single mutation induces a metal-dependent subunit association in dimeric Cu,Zn superoxide dismutase

D'ORAZIO, MELANIA;BATTISTONI, ANDREA;DESIDERI, ALESSANDRO
2000-01-01

Abstract

Tryptophan 83, a residue strongly involved in the intersubunit interaction of the Cu,Zn superoxide dismutases from Photobacterium leiognathi, has been selectively mutated to phenylalanine or tyrosine. The recombinant mutant enzymes expressed in Escherichia coil were purified in two well distinct and stable forms, one dimeric and fully active and the other monomeric and devoid of metals. In agreement, in vitro experiments indicate that the removal and addition of zinc in the mutant enzymes induces monomerization and dimerization, respectively, while does not perturb the dimeric association of the native protein. This is the first unambiguous experimental proof of a direct communication between the intersubunit interface and the metal active site. (C) 2000 Academic Press.
2000
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
Metalloproteins; Monomer-dimer equilibrium; Photobacterium leiognathi; Quaternary structure; Superoxide dismutase
D'Orazio, M., Battistoni, A., Stroppolo, M., Desideri, A. (2000). Single mutation induces a metal-dependent subunit association in dimeric Cu,Zn superoxide dismutase. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 272(1), 81-83 [10.1006/bbrc.2000.2730].
D'Orazio, M; Battistoni, A; Stroppolo, M; Desideri, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/55123
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