cDNA clones for Xenopus laevis Cu,Zn-superoxide dismutase were isolated, sequenced and used as probes to study the expression of the corresponding gene during oogenesis and embryogenesis; Cu,Zn-superoxide dismutase activity was also monitored throughout development. It has been observed that its mRNA is actively synthesized during early oogenesis, reaching a maximum level at stage II, and is utilized through oogenesis. This results in an accumulation of enzyme activity during oocyte growth, paralleling the accumulation of the several other cellular components which are stored in the oocyte to be utilized later on by the developing embryo. In fact, Cu,Zn-superoxide dismutase activity is present at an approximately constant level until late embryonic development, while its mRNA disappears soon after fertilization to be accumulated again only during the last part of embryogenesis. This developmental expression behaviour can be viewed as typical of an housekeeping function and suggests that Cu,Zn-superoxide dismutase activity is a constant need of the cell rather than being subject to regulation by oxygen metabolism.

Montesano, L., Carri', M.t., Mariottini, P., Amaldi, F., Rotilio, G. (1989). Developmental expression of Cu,Zn superoxide dismutase in Xenopus. Constant level of the enzyme in oogenesis and embryogenesis. EUROPEAN JOURNAL OF BIOCHEMISTRY, 186(1-2), 421-426.

Developmental expression of Cu,Zn superoxide dismutase in Xenopus. Constant level of the enzyme in oogenesis and embryogenesis

CARRI', MARIA TERESA;AMALDI, FRANCESCO;ROTILIO, GIUSEPPE
1989-12-08

Abstract

cDNA clones for Xenopus laevis Cu,Zn-superoxide dismutase were isolated, sequenced and used as probes to study the expression of the corresponding gene during oogenesis and embryogenesis; Cu,Zn-superoxide dismutase activity was also monitored throughout development. It has been observed that its mRNA is actively synthesized during early oogenesis, reaching a maximum level at stage II, and is utilized through oogenesis. This results in an accumulation of enzyme activity during oocyte growth, paralleling the accumulation of the several other cellular components which are stored in the oocyte to be utilized later on by the developing embryo. In fact, Cu,Zn-superoxide dismutase activity is present at an approximately constant level until late embryonic development, while its mRNA disappears soon after fertilization to be accumulated again only during the last part of embryogenesis. This developmental expression behaviour can be viewed as typical of an housekeeping function and suggests that Cu,Zn-superoxide dismutase activity is a constant need of the cell rather than being subject to regulation by oxygen metabolism.
8-dic-1989
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
Animals; Protein Biosynthesis; Superoxide Dismutase; Amino Acid Sequence; Embryo, Nonmammalian; Gene Amplification; RNA, Messenger; DNA, Circular; DNA Probes; Xenopus laevis; Molecular Sequence Data; Gene Expression Regulation; Oogenesis
Montesano, L., Carri', M.t., Mariottini, P., Amaldi, F., Rotilio, G. (1989). Developmental expression of Cu,Zn superoxide dismutase in Xenopus. Constant level of the enzyme in oogenesis and embryogenesis. EUROPEAN JOURNAL OF BIOCHEMISTRY, 186(1-2), 421-426.
Montesano, L; Carri', Mt; Mariottini, P; Amaldi, F; Rotilio, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/53357
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