The imbalance of the redox state in cells and body fluids in HIV-1- infected patients may result in progression of the disease as well as in immunologic disfunctions. In this report, we have evaluated whether the direct administration of high doses of reduced glutathione (GSH) exerts any antiviral activity and/or improves immune functions in a murine immunodeficiency animal model. Intramuscular administration of 50 or 100 mg GSH/mouse for five consecutive days weekly to LP-BM5-infected mice did not show local or systemic signs of acute toxicity. During the first 3 weeks from infection, a period in which clinical signs of disease were not yet detectable, GSH significantly reduced the viral load in lymph nodes and spleen as evaluated by a PCR semiquantitative assay of the proviral DNA content. At 10 weeks a GSH concentration-dependent reduction of splenomegaly, lymphadenopathy and hypergammaglobulinemia was evident in all treated mice. Evaluation of proviral DNA content showed that GSH was effective in inhibiting LP-BM5 infectivity in lymph nodes, spleen, and bone marrow at 100 mg/day, while it was less effective when administered at 50 mg/day. At 10 weeks some animals receiving the highest GSH dose died, thus only the mice receiving 50 mg GSH were followed up to 15 weeks without signs of toxicity. In this case, almost not significant differences among infected untreated or treated animals were observed. Thus, GSH is effective in reducing the proviral DNA load in the first period of infection. These data and the failure of sulfhydryl supplementation to further counteract the progression of disease after 10 weeks of infection suggest that combinations of GSH and other antiviral agents may be useful for improving current antiviral therapies.

Palamara, A.t., Garaci, E., Rotilio, G., Ciriolo, M.r., Casabianca, A., Fraternale, A., et al. (1996). Inhibition of murine AIDS by reduced glutathione. AIDS RESEARCH AND HUMAN RETROVIRUSES.

Inhibition of murine AIDS by reduced glutathione

GARACI, ENRICO;ROTILIO, GIUSEPPE;CIRIOLO, MARIA ROSA;
1996-01-01

Abstract

The imbalance of the redox state in cells and body fluids in HIV-1- infected patients may result in progression of the disease as well as in immunologic disfunctions. In this report, we have evaluated whether the direct administration of high doses of reduced glutathione (GSH) exerts any antiviral activity and/or improves immune functions in a murine immunodeficiency animal model. Intramuscular administration of 50 or 100 mg GSH/mouse for five consecutive days weekly to LP-BM5-infected mice did not show local or systemic signs of acute toxicity. During the first 3 weeks from infection, a period in which clinical signs of disease were not yet detectable, GSH significantly reduced the viral load in lymph nodes and spleen as evaluated by a PCR semiquantitative assay of the proviral DNA content. At 10 weeks a GSH concentration-dependent reduction of splenomegaly, lymphadenopathy and hypergammaglobulinemia was evident in all treated mice. Evaluation of proviral DNA content showed that GSH was effective in inhibiting LP-BM5 infectivity in lymph nodes, spleen, and bone marrow at 100 mg/day, while it was less effective when administered at 50 mg/day. At 10 weeks some animals receiving the highest GSH dose died, thus only the mice receiving 50 mg GSH were followed up to 15 weeks without signs of toxicity. In this case, almost not significant differences among infected untreated or treated animals were observed. Thus, GSH is effective in reducing the proviral DNA load in the first period of infection. These data and the failure of sulfhydryl supplementation to further counteract the progression of disease after 10 weeks of infection suggest that combinations of GSH and other antiviral agents may be useful for improving current antiviral therapies.
1996
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
English
Con Impact Factor ISI
glutathione; animal experiment; animal model; antiviral activity; article; clinical feature; concentration response; dna content; drug megadose; hypergammaglobulinemia; lymphadenopathy; mouse; nonhuman; oxidation reduction reaction; priority journal; rodent disease; splenomegaly; Acquired Immunodeficiency Syndrome; Animals; B-Lymphocytes; Disease Models, Animal; DNA, Viral; Glutathione; Hypergammaglobulinemia; Mice; Oxidation-Reduction; Polymerase Chain Reaction; T-Lymphocytes
Palamara, A.t., Garaci, E., Rotilio, G., Ciriolo, M.r., Casabianca, A., Fraternale, A., et al. (1996). Inhibition of murine AIDS by reduced glutathione. AIDS RESEARCH AND HUMAN RETROVIRUSES.
Palamara, At; Garaci, E; Rotilio, G; Ciriolo, Mr; Casabianca, A; Fraternale, A; Rossi, L; Schiavano, Gf; Chiarantini, L; Magnani, M
Articolo su rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/51980
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