Anaerobic treatment with cyanide of reduced ascorbate oxidase causes total depletion of copper. No significant amount of the metal is reincorporated when the apo-enzyme is incubated with cupric ions, but it is upon incubation with a stoichiometric amount (8 mol per mol of native enzyme) of a Cu(I) complex stable in air [Cu(I)(thiourea)3]Cl. The yield in reconstituted protein is higher under anaerobic conditions (85-90%) than in air (70-75%). By treatment with less than stoichiometric amounts of [Cu(I)(thiourea)3]Cl the apo-protein binds copper preferentially at the blue copper site. As a consequence the recovery of enzymatic activity is percentually lower than copper reincorporation.

Savini, I., Morpurgo, L., Avigliano, L. (1985). Full, reversible copper removal from ascorbate oxidase. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 131(3), 1251-1255.

Full, reversible copper removal from ascorbate oxidase

SAVINI, ISABELLA;AVIGLIANO, LUCIANA
1985-01-01

Abstract

Anaerobic treatment with cyanide of reduced ascorbate oxidase causes total depletion of copper. No significant amount of the metal is reincorporated when the apo-enzyme is incubated with cupric ions, but it is upon incubation with a stoichiometric amount (8 mol per mol of native enzyme) of a Cu(I) complex stable in air [Cu(I)(thiourea)3]Cl. The yield in reconstituted protein is higher under anaerobic conditions (85-90%) than in air (70-75%). By treatment with less than stoichiometric amounts of [Cu(I)(thiourea)3]Cl the apo-protein binds copper preferentially at the blue copper site. As a consequence the recovery of enzymatic activity is percentually lower than copper reincorporation.
1985
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
ascorbate oxidase; copper; nonhuman; plant; priority journal; Apoenzymes; Apoproteins; Ascorbate Oxidase; Chemistry; Copper; Cyanides; Electron Spin Resonance Spectroscopy; Oxidoreductases; Oxygen; Spectrophotometry; Thiourea
Savini, I., Morpurgo, L., Avigliano, L. (1985). Full, reversible copper removal from ascorbate oxidase. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 131(3), 1251-1255.
Savini, I; Morpurgo, L; Avigliano, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/51162
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