Increasing evidence suggests that regulation of apoptosis in infected cells is associated with several viral infections. The gammaherpesvirus bovine herpesvirus 4 (BHV-4) has been shown to harbor genes with antiapoptotic potentialities. However, here we have demonstrated that productive infection of adherent, permissive cell lines by BHV-4 resulted in a cytopathic effect characterized by induction of apoptosis. This phenomenon was confirmed using different techniques to detect apoptosis and using different virus strains and cell targets. Apoptosis induced by BHV-4 was inhibited by (1) treatment with doses of heparin, which completely inhibited virus attachment and infectivity; (2) UV treatment, which completely abrogated infectivity; and (3) treatment with a dose of phosphonoacetic acid, which blocked virus replication. Virus-induced apoptosis was associated with a down-regulation of Bcl-2 expression and was reduced by Z-VAD-FMK, but not by Z-DEVD-FMK (caspase-3-specific) caspase inhibitors. Inhibition of apoptosis by Z-VAD-FMK treatment during infection did not modify virus yield. Therefore, despite the presence of antiapoptotic genes in its genoma, BHV-4 could complete its cycle of productive infection while inducing apoptosis of infected cells. This finding might have implications for the pathobiology of BHV-4 and other gammaherpesviruses in vivo, (C) 2000 Academic Press.

Sciortino, M., Perri, D., Medici, M., Foti, C., Orlandella, B., Mastino, A. (2000). The Gamma-2-herpesvirus bovine herpesvirus 4 causes apoptotic infection in permissive cell lines. VIROLOGY, 277(1), 27-39 [10.1006/viro.2000.0575].

The Gamma-2-herpesvirus bovine herpesvirus 4 causes apoptotic infection in permissive cell lines

FOTI, CALOGERO;
2000-01-01

Abstract

Increasing evidence suggests that regulation of apoptosis in infected cells is associated with several viral infections. The gammaherpesvirus bovine herpesvirus 4 (BHV-4) has been shown to harbor genes with antiapoptotic potentialities. However, here we have demonstrated that productive infection of adherent, permissive cell lines by BHV-4 resulted in a cytopathic effect characterized by induction of apoptosis. This phenomenon was confirmed using different techniques to detect apoptosis and using different virus strains and cell targets. Apoptosis induced by BHV-4 was inhibited by (1) treatment with doses of heparin, which completely inhibited virus attachment and infectivity; (2) UV treatment, which completely abrogated infectivity; and (3) treatment with a dose of phosphonoacetic acid, which blocked virus replication. Virus-induced apoptosis was associated with a down-regulation of Bcl-2 expression and was reduced by Z-VAD-FMK, but not by Z-DEVD-FMK (caspase-3-specific) caspase inhibitors. Inhibition of apoptosis by Z-VAD-FMK treatment during infection did not modify virus yield. Therefore, despite the presence of antiapoptotic genes in its genoma, BHV-4 could complete its cycle of productive infection while inducing apoptosis of infected cells. This finding might have implications for the pathobiology of BHV-4 and other gammaherpesviruses in vivo, (C) 2000 Academic Press.
2000
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/34 - MEDICINA FISICA E RIABILITATIVA
English
SARCOMA-ASSOCIATED HERPESVIRUS; FACTOR RECEPTOR-1-INDUCED APOPTOSIS; EPSTEIN-BARR-VIRUS; INHIBITS APOPTOSIS; BCL-2 HOMOLOG; BLOCKS APOPTOSIS; DEATH; PROTEIN; TYPE-4; REPLICATION
Sciortino, M., Perri, D., Medici, M., Foti, C., Orlandella, B., Mastino, A. (2000). The Gamma-2-herpesvirus bovine herpesvirus 4 causes apoptotic infection in permissive cell lines. VIROLOGY, 277(1), 27-39 [10.1006/viro.2000.0575].
Sciortino, M; Perri, D; Medici, M; Foti, C; Orlandella, B; Mastino, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/51132
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