Prostaglandin E2 (PGE2) is a known negative regulator of T lymphocyte proliferation. Previously we have indirectly evidentiated the involvement of PGE2 in apoptosis of lymphocytes both in vitro and in vivo. We have evaluated a possible direct effect of PGE2 on apoptosis. To this end we have investigated the in vitro effects of PGE2 on cell death, and its possible correlation with c-Myc and Bcl-2 proteins. We used freshly isolated unstimulated human lymphocytes from neonatal thymus, cord blood and adult peripheral blood. PGE2 induced DNA fragmentation in both peripheral and cord blood at 10-7 to 10-5 M concentrations, even though this induction was delayed in peripheral blood with respect to cord blood. Apoptosis induced by PGE2 was always associated with a dose-dependent increase of cellular steady state c-Myc protein levels, whereas Bcl-2 protein levels were not substantially affected. Unstimulated thymocytes showed spontaneous DNA fragmentation that occurred earlier and at higher levels in PGE2- (10-5 M) treated cells with respect to untreated controls. Also in these cells, PGE2 produced an early increase of c-Myc protein expression, although Bcl-2 protein levels remained unchanged. In conclusion, PGE2 induces apoptosis with different kinetics on immature and mature T cells: this induction is associated with the increase of c-Myc protein expression and seems to be independent from Bcl-2 regulation.

Pica, F., Franzese, O., D'Onofrio, C., Bonmassar, E., Favalli, C., Garaci, E. (1996). Prostaglandin E2 induces apoptosis in resting immature and mature human lymphocytes: a c-Myc-dependent and Bcl-2-independent associated pathway. THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 277(3), 1793-1800.

Prostaglandin E2 induces apoptosis in resting immature and mature human lymphocytes: a c-Myc-dependent and Bcl-2-independent associated pathway

PICA, FRANCESCA;FRANZESE, ORNELLA;FAVALLI, CARTESIO;GARACI, ENRICO
1996-01-01

Abstract

Prostaglandin E2 (PGE2) is a known negative regulator of T lymphocyte proliferation. Previously we have indirectly evidentiated the involvement of PGE2 in apoptosis of lymphocytes both in vitro and in vivo. We have evaluated a possible direct effect of PGE2 on apoptosis. To this end we have investigated the in vitro effects of PGE2 on cell death, and its possible correlation with c-Myc and Bcl-2 proteins. We used freshly isolated unstimulated human lymphocytes from neonatal thymus, cord blood and adult peripheral blood. PGE2 induced DNA fragmentation in both peripheral and cord blood at 10-7 to 10-5 M concentrations, even though this induction was delayed in peripheral blood with respect to cord blood. Apoptosis induced by PGE2 was always associated with a dose-dependent increase of cellular steady state c-Myc protein levels, whereas Bcl-2 protein levels were not substantially affected. Unstimulated thymocytes showed spontaneous DNA fragmentation that occurred earlier and at higher levels in PGE2- (10-5 M) treated cells with respect to untreated controls. Also in these cells, PGE2 produced an early increase of c-Myc protein expression, although Bcl-2 protein levels remained unchanged. In conclusion, PGE2 induces apoptosis with different kinetics on immature and mature T cells: this induction is associated with the increase of c-Myc protein expression and seems to be independent from Bcl-2 regulation.
1996
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
English
Con Impact Factor ISI
DNA fragment; Myc protein; prostaglandin E2; oncoprotein; protein bcl 2; apoptosis; article; cell death; cell kinetics; controlled study; dose response; etiology; human; human cell; immunoblotting; lymphocyte culture; lymphocyte proliferation; oncogene c myc; priority journal; protein expression; regulatory mechanism; T lymphocyte; thymocyte; drug effect; gene expression; lymphocyte; oncogene myc; Apoptosis; Dinoprostone; Gene Expression; Genes, myc; Humans; Immunoblotting; Lymphocytes; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-bcl-2
Pica, F., Franzese, O., D'Onofrio, C., Bonmassar, E., Favalli, C., Garaci, E. (1996). Prostaglandin E2 induces apoptosis in resting immature and mature human lymphocytes: a c-Myc-dependent and Bcl-2-independent associated pathway. THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 277(3), 1793-1800.
Pica, F; Franzese, O; D'Onofrio, C; Bonmassar, E; Favalli, C; Garaci, E
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/50867
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