Four different human tumor cell lines of glial origin have been exposed to a human T lymphotropic retrovirus (HTLV-I). All these cell lines were positive for the glial marker glial fibrillary acidic protein (GFAP). The presence of virus RNA was demonstrated by in situ hybridization using an HTLV-I, SStI-SStI viral insert as probe. Virus expression has been monitored through an indirect immunofluorescence assay using a monoclonal antibody against virus core protein p19. All the four glioma cell lines tested became positive for p19 after 2 weeks of co-cultivation and showed a clear alteration of GFAP expression.
Macchi, B., Caronti, B., Pezzella, M., Bonmassar, E., Lauro, G. (1991). Effect of human T lymphotropic retrovirus-I exposure on cultured human glioma cell lines. ACTA NEUROPATHOLOGICA(6), 670-674.
Effect of human T lymphotropic retrovirus-I exposure on cultured human glioma cell lines
MACCHI, BEATRICE;
1991-01-01
Abstract
Four different human tumor cell lines of glial origin have been exposed to a human T lymphotropic retrovirus (HTLV-I). All these cell lines were positive for the glial marker glial fibrillary acidic protein (GFAP). The presence of virus RNA was demonstrated by in situ hybridization using an HTLV-I, SStI-SStI viral insert as probe. Virus expression has been monitored through an indirect immunofluorescence assay using a monoclonal antibody against virus core protein p19. All the four glioma cell lines tested became positive for p19 after 2 weeks of co-cultivation and showed a clear alteration of GFAP expression.Questo articolo è pubblicato sotto una Licenza Licenza Creative Commons
