Gangliosides participate in development and tissue differentiation, Cross-linking of the apoptosis-inducing CD95 protein (also called Fas or APO-1) in lymphoid and myeloid tumor cells triggered GD3 ganglioside synthesis and transient accumulation. CD95-induced GD3 accumulation depended on integral receptor ''death domains'' and on activation of a family of cysteine proteases called caspases. Cell-permeating ceramides, which are potent inducers of apoptosis, also triggered GD3 synthesis, GD3 disrupted mitochondrial transmembrane potential (Delta psi(m),), and induced apoptosis, in a caspase-independent fashion. Transient overexpression of the GD3 synthase gene directly triggered apoptosis, Pharmacological inhibition of GD3 synthesis and exposure to GD3 synthase antisense oligodeoxynucleotides prevented CD95-induced apoptosis. Thus, GD3 ganglioside mediates the propagation of CD95-generated apoptotic signals in hematopoietic cells.

De Maria, R., Lenti, L., Malisan, F., D'Agostino, F., Tomassini, B., Zeuner, A., et al. (1997). Requirement for GD3 ganglioside in CD95- and ceramide-induced apoptosis. SCIENCE, 277(5332), 1652-1655 [10.1126/science.277.5332.1652].

Requirement for GD3 ganglioside in CD95- and ceramide-induced apoptosis

MALISAN, FLORENCE;TESTI, ROBERTO
1997-01-01

Abstract

Gangliosides participate in development and tissue differentiation, Cross-linking of the apoptosis-inducing CD95 protein (also called Fas or APO-1) in lymphoid and myeloid tumor cells triggered GD3 ganglioside synthesis and transient accumulation. CD95-induced GD3 accumulation depended on integral receptor ''death domains'' and on activation of a family of cysteine proteases called caspases. Cell-permeating ceramides, which are potent inducers of apoptosis, also triggered GD3 synthesis, GD3 disrupted mitochondrial transmembrane potential (Delta psi(m),), and induced apoptosis, in a caspase-independent fashion. Transient overexpression of the GD3 synthase gene directly triggered apoptosis, Pharmacological inhibition of GD3 synthesis and exposure to GD3 synthase antisense oligodeoxynucleotides prevented CD95-induced apoptosis. Thus, GD3 ganglioside mediates the propagation of CD95-generated apoptotic signals in hematopoietic cells.
1997
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/04 - PATOLOGIA GENERALE
English
Con Impact Factor ISI
FAS-MEDIATED APOPTOSIS; ACIDIC SPHINGOMYELINASE; EXPRESSION CLONING; G(D3) SYNTHASE; CELL-DEATH; PROTEASE; INVOLVEMENT; RECEPTOR; SIGNAL; MEMBRANE
De Maria, R., Lenti, L., Malisan, F., D'Agostino, F., Tomassini, B., Zeuner, A., et al. (1997). Requirement for GD3 ganglioside in CD95- and ceramide-induced apoptosis. SCIENCE, 277(5332), 1652-1655 [10.1126/science.277.5332.1652].
De Maria, R; Lenti, L; Malisan, F; D'Agostino, F; Tomassini, B; Zeuner, A; Rippo, Mr; Testi, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/49433
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