The regulation of the synthesis of elongation factor 1 alpha (EF-1 alpha) in Xenopus laevis has been analyzed from the point of view of translational control. The 5' end of EF-1 alpha mRNA, examined by primer extension, revealed the presence of a terminal pyrimidine tract that is characteristic of ribosomal protein mRNAs (rp-mRNAs). We have then compared the translation pattern of EF-1 alpha and rp-mRNAs during Xenopus embryogenesis and in Xenopus cultured cells during growth rate changes. In Xenopus embryos EF-1 alpha transcripts, that appear after midblastula transition, are initially mostly localized on mRNP and translationally inactive. Only later in embryogenesis, together with rp-mRNAs, they are gradually recruited on polysomes. Also in Xenopus cells B 3.2, EF-1 alpha mRNA shows a distribution change similar to an rp-mRNA: part of it moves from polysomes to mRNP during serum deprivation and goes back on polysomes after restitution of serum to the culture. Moreover EF-1 alpha mRNA, similarly to rp-mRNAs, is always localized on mRNP or fully loaded on polysomes but never on small polysomes. Therefore EF-1 alpha mRNA for structural features and translation behavior can be included in the 'regulatory' group of rp-mRNAs.

Loreni, F., Francesconi, A., Amaldi, F. (1993). Coordinate translational regulation in the syntheses of elongation factor 1 alpha and ribosomal proteins in Xenopus laevis. NUCLEIC ACIDS RESEARCH, 21(20), 4721-4725.

Coordinate translational regulation in the syntheses of elongation factor 1 alpha and ribosomal proteins in Xenopus laevis

LORENI, FABRIZIO;AMALDI, FRANCESCO
1993-01-01

Abstract

The regulation of the synthesis of elongation factor 1 alpha (EF-1 alpha) in Xenopus laevis has been analyzed from the point of view of translational control. The 5' end of EF-1 alpha mRNA, examined by primer extension, revealed the presence of a terminal pyrimidine tract that is characteristic of ribosomal protein mRNAs (rp-mRNAs). We have then compared the translation pattern of EF-1 alpha and rp-mRNAs during Xenopus embryogenesis and in Xenopus cultured cells during growth rate changes. In Xenopus embryos EF-1 alpha transcripts, that appear after midblastula transition, are initially mostly localized on mRNP and translationally inactive. Only later in embryogenesis, together with rp-mRNAs, they are gradually recruited on polysomes. Also in Xenopus cells B 3.2, EF-1 alpha mRNA shows a distribution change similar to an rp-mRNA: part of it moves from polysomes to mRNP during serum deprivation and goes back on polysomes after restitution of serum to the culture. Moreover EF-1 alpha mRNA, similarly to rp-mRNAs, is always localized on mRNP or fully loaded on polysomes but never on small polysomes. Therefore EF-1 alpha mRNA for structural features and translation behavior can be included in the 'regulatory' group of rp-mRNAs.
1993
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/11 - BIOLOGIA MOLECOLARE
English
Con Impact Factor ISI
Protein Biosynthesis; Animals; Blotting, Northern; Transcription, Genetic; Peptide Elongation Factors; Ribosomal Proteins; RNA, Messenger; Xenopus laevis; Base Sequence; Cells, Cultured; Molecular Sequence Data; Peptide Elongation Factor 1; Gene Expression Regulation
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC331496/
Loreni, F., Francesconi, A., Amaldi, F. (1993). Coordinate translational regulation in the syntheses of elongation factor 1 alpha and ribosomal proteins in Xenopus laevis. NUCLEIC ACIDS RESEARCH, 21(20), 4721-4725.
Loreni, F; Francesconi, A; Amaldi, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/48248
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