The nucleolar localization elements (NoLEs) of U17 small nucleolar RNA (snoRNA), which is essential for rRNA processing and belongs to the box H/ACA snoRNA family, were analyzed by fluorescence microscopy. Injection of mutant U17 transcripts into Xenopus laevis oocyte nuclei revealed that deletion of stems 1, 2, and 4 of U17 snoRNA reduced but did not prevent nucleolar localization. The deletion of stem 3 had no adverse effect. Therefore, the hairpins of the hairpin-hinge-hairpin-tail structure formed by these stems are not absolutely critical for nucleolar localization of U17, nor are sequences within stems 1, 3, and 4, which may tether U17 to the rRNA precursor by base pairing. In contrast, box H and box ACA are major NoLEs; their combined substitution or deletion abolished nucleolar localization of U17 snoRNA. Mutation of just box H or just the box ACA region alone did not fully abolish the nucleolar localization of U17. This indicates that the NoLEs of the box H/ACA snoRNA family function differently from the bipartite NoLEs (conserved boxes C and D) of box C/D snoRNAs, where mutation of either box alone prevents nucleolar localization.

Lange, T., Ezrokhi, M., Amaldi, F., Gerbi, S. (1999). Box H and box ACA are nucleolar localization elements of U17 small nucleolar RNA. MOLECULAR BIOLOGY OF THE CELL, 10(11), 3877-3890.

Box H and box ACA are nucleolar localization elements of U17 small nucleolar RNA

AMALDI, FRANCESCO;
1999-01-01

Abstract

The nucleolar localization elements (NoLEs) of U17 small nucleolar RNA (snoRNA), which is essential for rRNA processing and belongs to the box H/ACA snoRNA family, were analyzed by fluorescence microscopy. Injection of mutant U17 transcripts into Xenopus laevis oocyte nuclei revealed that deletion of stems 1, 2, and 4 of U17 snoRNA reduced but did not prevent nucleolar localization. The deletion of stem 3 had no adverse effect. Therefore, the hairpins of the hairpin-hinge-hairpin-tail structure formed by these stems are not absolutely critical for nucleolar localization of U17, nor are sequences within stems 1, 3, and 4, which may tether U17 to the rRNA precursor by base pairing. In contrast, box H and box ACA are major NoLEs; their combined substitution or deletion abolished nucleolar localization of U17 snoRNA. Mutation of just box H or just the box ACA region alone did not fully abolish the nucleolar localization of U17. This indicates that the NoLEs of the box H/ACA snoRNA family function differently from the bipartite NoLEs (conserved boxes C and D) of box C/D snoRNAs, where mutation of either box alone prevents nucleolar localization.
1999
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/11 - BIOLOGIA MOLECOLARE
English
Con Impact Factor ISI
small nuclear RNA; animal cell; article; fluorescence microscopy; gene deletion; nonhuman; oocyte; priority journal; protein localization; protein stability; RNA processing; structure analysis; xenopus laevis; Animals; Base Sequence; Cell Nucleolus; Conserved Sequence; Evolution, Molecular; Microinjections; Microscopy, Fluorescence; Molecular Sequence Data; Mutation; Nucleic Acid Conformation; Oocytes; RNA, Nuclear; RNA, Ribosomal; RNA, Small Nucleolar; Sequence Deletion; Xenopus
Lange, T., Ezrokhi, M., Amaldi, F., Gerbi, S. (1999). Box H and box ACA are nucleolar localization elements of U17 small nucleolar RNA. MOLECULAR BIOLOGY OF THE CELL, 10(11), 3877-3890.
Lange, T; Ezrokhi, M; Amaldi, F; Gerbi, S
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/48139
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