The effect of X-ray irradiation on cell survival, induction, and repair of DNA damage was studied by using 10 Chroococcidiopsis strains isolated from desert and hypersaline environments. After exposure to 2.5 kGy, the percentages of survival for the strains ranged from 80 to 35%. In the four most resistant strains, the levels of survival were reduced by 1 or 2 orders of magnitude after irradiation with 5 kGy; viable cells were recovered after exposure to 15 kGy but not after exposure to 20 kGy. The severe DNA damage evident after exposure to 2.5 kGy was repaired within 3 h, and the severe DNA damage evident after exposure to 5 kGy was repaired within 24 h. The increase in trichloroacetic acid-precipitable radioactivity in the culture supernatant after irradiation with 2.5 kGy might have been due to cell lysis and/or an excision process involved in DNA repair. The radiation resistance of Chroococcidiopsis strains may reflect the ability of these cyanobacteria to survive prolonged desiccation through efficient repair of the DNA damage that accumulates during dehydration.

Billi, D., Friedmann, E., Hofer, K., Grilli Caiola, M., Ocampo Friedmann, R. (2000). Ionizing-radiation resistance in the desiccation-tolerant cyanobacterium Chroococcidiopsis. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 66(4), 1489-1492 [10.1128/AEM.66.4.1489-1492.2000].

Ionizing-radiation resistance in the desiccation-tolerant cyanobacterium Chroococcidiopsis

BILLI, DANIELA;
2000-01-01

Abstract

The effect of X-ray irradiation on cell survival, induction, and repair of DNA damage was studied by using 10 Chroococcidiopsis strains isolated from desert and hypersaline environments. After exposure to 2.5 kGy, the percentages of survival for the strains ranged from 80 to 35%. In the four most resistant strains, the levels of survival were reduced by 1 or 2 orders of magnitude after irradiation with 5 kGy; viable cells were recovered after exposure to 15 kGy but not after exposure to 20 kGy. The severe DNA damage evident after exposure to 2.5 kGy was repaired within 3 h, and the severe DNA damage evident after exposure to 5 kGy was repaired within 24 h. The increase in trichloroacetic acid-precipitable radioactivity in the culture supernatant after irradiation with 2.5 kGy might have been due to cell lysis and/or an excision process involved in DNA repair. The radiation resistance of Chroococcidiopsis strains may reflect the ability of these cyanobacteria to survive prolonged desiccation through efficient repair of the DNA damage that accumulates during dehydration.
2000
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/01 - BOTANICA GENERALE
English
Con Impact Factor ISI
DNA; article; bacterial growth; colony forming unit; Cyanobacterium; dehydration; desiccation; DNA damage; ionizing radiation; nonhuman; radiation exposure; survival; NASA Discipline Exobiology; Non-NASA Center; Cyanobacteria; Desiccation; DNA Damage; DNA Repair; Radiation Dosage; Radiation Tolerance; Radiation, Ionizing; X-Rays; Bacteria (microorganisms); Chroococcidiopsis; Cyanobacteria
Billi, D., Friedmann, E., Hofer, K., Grilli Caiola, M., Ocampo Friedmann, R. (2000). Ionizing-radiation resistance in the desiccation-tolerant cyanobacterium Chroococcidiopsis. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 66(4), 1489-1492 [10.1128/AEM.66.4.1489-1492.2000].
Billi, D; Friedmann, E; Hofer, K; Grilli Caiola, M; Ocampo Friedmann, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/41227
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