Eight primers were developed for the amplification of mitochondrial DNA control region of Old world porcupines (subgenus Hystrix). Successful amplifications of low-quality DNA extracted from old (12 years old) and recent quills were performed, thus facilitating field sampling. Successful cross-species amplifications were obtained for Hystrix africaeaustralis, H. cristata and H. indica. Length and structure of mitochondrial DNA control region were analysed and its usefulness as genetic marker for interspecific and population investigation was discussed.
Trucchi, E., Gentile, G., Sbordoni, V. (2008). Development of primers to amplify mitochondrial DNA control region of Old World porcupines (subgenus Hystrix). MOLECULAR ECOLOGY RESOURCES, 8(5), 1139-1141 [10.1111/j.1755-0998.2008.02201.x].
Development of primers to amplify mitochondrial DNA control region of Old World porcupines (subgenus Hystrix)
GENTILE, GABRIELE;SBORDONI, VALERIO
2008-01-01
Abstract
Eight primers were developed for the amplification of mitochondrial DNA control region of Old world porcupines (subgenus Hystrix). Successful amplifications of low-quality DNA extracted from old (12 years old) and recent quills were performed, thus facilitating field sampling. Successful cross-species amplifications were obtained for Hystrix africaeaustralis, H. cristata and H. indica. Length and structure of mitochondrial DNA control region were analysed and its usefulness as genetic marker for interspecific and population investigation was discussed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
