With the aim of identifying the immunophenotypic profile of B-cell chronic lymphocytic leukemia (B-CLL) subsets with different prognosis, we investigated by flow cytometry the expression of 36 surface antigens in 123 cases, all with survivals. By analyzing results with unsupervised (hierarchical and K-means clustering) algorithms, three distinct immunophenotypic groups (I, II, and III) were identified, group I (51/123) with longer survivals, as compared to the group II (36/123) and III (36/123). The immunophenotypic signatures of these groups, as determined by applying the nearest Shrunken centroids method as class predictor, were characterized by the coordinated and differential expression of 12 surface markers, that is, group I: above-average expression of CD62L, CD54, CD49c, and CD25, below-average expression of CD38; group II: above-average expression of CD38, CD49d, CD29, and CD49e; and group III: below-average expression of the above markers, overexpression of CD23, CD20, SmIg, and CD79b. As opposed to groups II-III, group I B-CLLs lacked expression of ZAP-70 and activation-induced cytidine deaminase in the majority of cases, while more frequently had mutated IgV(H) genes and IgV(H) mutations consistent with antigen-driven selection. Our findings contribute to improve the immunophenotypical identification of disease subsets with different prognosis and suggest a set of surface antigens to be employed as prognosticators in routine diagnostic/prognostic procedures.

Zucchetto, A., Sonego, P., Degan, M., Bomben, R., Dal Bo, M., Russo, S., et al. (2005). Signature of B-CLL with different prognosis by Shrunken centroids of surface antigen expression profiling, 204(1), 113-123 [10.1002/jcp.20269].

Signature of B-CLL with different prognosis by Shrunken centroids of surface antigen expression profiling

BUCCISANO, FRANCESCO;DEL PRINCIPE, MARIA ILARIA;DEL POETA, GIOVANNI;
2005-07-01

Abstract

With the aim of identifying the immunophenotypic profile of B-cell chronic lymphocytic leukemia (B-CLL) subsets with different prognosis, we investigated by flow cytometry the expression of 36 surface antigens in 123 cases, all with survivals. By analyzing results with unsupervised (hierarchical and K-means clustering) algorithms, three distinct immunophenotypic groups (I, II, and III) were identified, group I (51/123) with longer survivals, as compared to the group II (36/123) and III (36/123). The immunophenotypic signatures of these groups, as determined by applying the nearest Shrunken centroids method as class predictor, were characterized by the coordinated and differential expression of 12 surface markers, that is, group I: above-average expression of CD62L, CD54, CD49c, and CD25, below-average expression of CD38; group II: above-average expression of CD38, CD49d, CD29, and CD49e; and group III: below-average expression of the above markers, overexpression of CD23, CD20, SmIg, and CD79b. As opposed to groups II-III, group I B-CLLs lacked expression of ZAP-70 and activation-induced cytidine deaminase in the majority of cases, while more frequently had mutated IgV(H) genes and IgV(H) mutations consistent with antigen-driven selection. Our findings contribute to improve the immunophenotypical identification of disease subsets with different prognosis and suggest a set of surface antigens to be employed as prognosticators in routine diagnostic/prognostic procedures.
lug-2005
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/15 - MALATTIE DEL SANGUE
English
Con Impact Factor ISI
Antigens, Surface; Humans; Prognosis; Protein-Tyrosine Kinases; Aged; Cytidine Deaminase; Aged, 80 and over; Leukemia, Lymphocytic, Chronic, B-Cell; Adult; Immunoglobulin Variable Region; Middle Aged; Flow Cytometry; Cytosine Deaminase; Tumor Markers, Biological; Cluster Analysis; Immunophenotyping; Male; Female; ZAP-70 Protein-Tyrosine Kinase; Survival Analysis
Zucchetto, A., Sonego, P., Degan, M., Bomben, R., Dal Bo, M., Russo, S., et al. (2005). Signature of B-CLL with different prognosis by Shrunken centroids of surface antigen expression profiling, 204(1), 113-123 [10.1002/jcp.20269].
Zucchetto, A; Sonego, P; Degan, M; Bomben, R; Dal Bo, M; Russo, S; Attadia, V; Rupolo, M; Buccisano, F; DEL PRINCIPE, Mi; DEL POETA, G; Pucillo, C; Colombatti, A; Campanini, R; Gattei, V
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/39969
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