A simple and reliable technique has been developed for the construction of an amperometric acetylcholinesterase biosensor based on screen-printed carbon electrodes. For the first time, one-step modification using single-walled carbon nanotubes and Co phtalocyanine has been proposed to decrease the working potential and to increase the signal of thiocholine oxidation. The biosensor developed made it possible to detect 5-50 ppb of paraoxon and 2-50 ppb of malaoxon with detection limits of 3 and 2 ppb, respectively (incubation 15 min). The biosensor showed high reproducibility when measurements of the substrate and inhibitor were performed (R.S.D. about 1% and 2.5%, respectively). The reliability of the inhibition measurements was confirmed by testing spiked samples of sparkling and tape waters. © 2011 Elsevier B.V. All rights reserved.
Ivanov, A., Younusov, R., Evtugyn, G., Arduini, F., MOSCONE DINIA, D., Palleschi, G. (2011). Acetylcholinesterase biosensor based on single-walled carbon nanotubes - Co phtalocyanine for organophosphorus pesticides detection. TALANTA, 85(1), 216-221 [10.1016/j.talanta.2011.03.045].
Acetylcholinesterase biosensor based on single-walled carbon nanotubes - Co phtalocyanine for organophosphorus pesticides detection
ARDUINI, FABIANA;MOSCONE DINIA, DANILA;PALLESCHI, GIUSEPPE
2011-01-01
Abstract
A simple and reliable technique has been developed for the construction of an amperometric acetylcholinesterase biosensor based on screen-printed carbon electrodes. For the first time, one-step modification using single-walled carbon nanotubes and Co phtalocyanine has been proposed to decrease the working potential and to increase the signal of thiocholine oxidation. The biosensor developed made it possible to detect 5-50 ppb of paraoxon and 2-50 ppb of malaoxon with detection limits of 3 and 2 ppb, respectively (incubation 15 min). The biosensor showed high reproducibility when measurements of the substrate and inhibitor were performed (R.S.D. about 1% and 2.5%, respectively). The reliability of the inhibition measurements was confirmed by testing spiked samples of sparkling and tape waters. © 2011 Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
