Osteoarthritis is a progressive joint disease characterized by cartilage degradation and bone remodeling. Transglutaminases catalyze a calcium-dependent transamidation reaction that produces covalent cross-linking of available substrate glutamine residues and modifies the extracellular matrix. Increased transglutaminases-mediated activity is reported in osteoarthritis, but the relative contribution of transglutaminases-2 (TG2) is uncertain. We describe TG2 expression in human femoral osteoarthritis and in wild-type and homozygous TG2 knockout mice after surgically-induced knee joint instability. Increased TG2 levels were observed in human and wild-type murine osteoarthritic cartilage compared to the respective controls. Histomorphometrical but not X-ray investigation documented in osteoarthritic TG2 knockout mice reduced cartilage destruction and an increased osteophyte formation compared to wild-type mice. These differences were associated with increased TGF beta-1 expression. In addition to confirming its important role in osteoarthritis development, our results demonstrated that TG2 expression differently influences cartilage destruction and bone remodeling, suggesting new targeted TG2-related therapeutic strategies.

Orlandi, A., Oliva, F., Taurisano, G., Candi, E., Di Lascio, A., Melino, G., et al. (2009). Transglutaminase-2 differently regulates cartilage destruction and osteophyte formation in a surgical model of osteoarthritis. In AMINO ACIDS (pp.755-763). new York : Springer [10.1007/s00726-008-0129-3].

Transglutaminase-2 differently regulates cartilage destruction and osteophyte formation in a surgical model of osteoarthritis

ORLANDI, AUGUSTO;CANDI, ELEONORA;MELINO, GENNARO;SPAGNOLI, LUIGI GIUSTO;TARANTINO, UMBERTO
2009-01-01

Abstract

Osteoarthritis is a progressive joint disease characterized by cartilage degradation and bone remodeling. Transglutaminases catalyze a calcium-dependent transamidation reaction that produces covalent cross-linking of available substrate glutamine residues and modifies the extracellular matrix. Increased transglutaminases-mediated activity is reported in osteoarthritis, but the relative contribution of transglutaminases-2 (TG2) is uncertain. We describe TG2 expression in human femoral osteoarthritis and in wild-type and homozygous TG2 knockout mice after surgically-induced knee joint instability. Increased TG2 levels were observed in human and wild-type murine osteoarthritic cartilage compared to the respective controls. Histomorphometrical but not X-ray investigation documented in osteoarthritic TG2 knockout mice reduced cartilage destruction and an increased osteophyte formation compared to wild-type mice. These differences were associated with increased TGF beta-1 expression. In addition to confirming its important role in osteoarthritis development, our results demonstrated that TG2 expression differently influences cartilage destruction and bone remodeling, suggesting new targeted TG2-related therapeutic strategies.
9th Conference on Transglutaminase and Protein Crosslinking
Marrakesh, MONACO
SEP, 2007
Rilevanza internazionale
2009
Settore MED/08 - ANATOMIA PATOLOGICA
English
Osteoarthritis; Transglutaminase-2; Cartilage; Osteophyte; TGF-beta 1
9
Intervento a convegno
Orlandi, A., Oliva, F., Taurisano, G., Candi, E., Di Lascio, A., Melino, G., et al. (2009). Transglutaminase-2 differently regulates cartilage destruction and osteophyte formation in a surgical model of osteoarthritis. In AMINO ACIDS (pp.755-763). new York : Springer [10.1007/s00726-008-0129-3].
Orlandi, A; Oliva, F; Taurisano, G; Candi, E; Di Lascio, A; Melino, G; Spagnoli, Lg; Tarantino, U
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/39162
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