The aim of the work was to evaluate the circulation of the viruses and to determine a correlation between faecal indicators and viruses. Raw wastewater and effluent samples were collected from three wastewater treatment plants, during three sampling periods, and analysed, using cultural and molecular methods, to determine bacteria and virus presence. The results show a removal of bacterial indicators, but a limited reduction of the phages. The viral analysis displays the circulation of cultivable enteroviruses and differences in the seasonal-geographical distribution. Hepatitis A virus was found with only two genotypes: IA-IB. Rotavirus was present in 11.11%, 24.14%, 2.78% of the samples in the 1st, 2nd and 3rd sampling periods; Astrovirus in 33.33%, 6.9%, 25%; Adenovirus in 7.41%, 3.45%, 2.78%; Norovirus in 7.41%, 10.34%, 5.56% respectively. Adenovirus was never identified in plants B and C as Rotavirus in plant C. The presence of faecal indicators was not predictive of the enteric virus presence, whereas a different circulation of Enteroviruses was found in the wastewater treatment plants. The study shows the importance and the usefulness of molecular methods to evaluate the virus circulation and the genetic variability of Enteroviruses.

Petrinca, A., Donia, D.t., Pierangeli, A., Gabrieli, R., Degener, A., Bonanni, E., et al. (2009). Presence and environmental circulation of enteric viruses in three different wastewater treatment plants. JOURNAL OF APPLIED MICROBIOLOGY, 106(5), 1608-1617 [10.1111/j.1365-2672.2008.04128.x].

Presence and environmental circulation of enteric viruses in three different wastewater treatment plants

DONIA, DOMENICA TOMMASA;GABRIELI, ROSANNA;DIVIZIA, MAURIZIO
2009-01-01

Abstract

The aim of the work was to evaluate the circulation of the viruses and to determine a correlation between faecal indicators and viruses. Raw wastewater and effluent samples were collected from three wastewater treatment plants, during three sampling periods, and analysed, using cultural and molecular methods, to determine bacteria and virus presence. The results show a removal of bacterial indicators, but a limited reduction of the phages. The viral analysis displays the circulation of cultivable enteroviruses and differences in the seasonal-geographical distribution. Hepatitis A virus was found with only two genotypes: IA-IB. Rotavirus was present in 11.11%, 24.14%, 2.78% of the samples in the 1st, 2nd and 3rd sampling periods; Astrovirus in 33.33%, 6.9%, 25%; Adenovirus in 7.41%, 3.45%, 2.78%; Norovirus in 7.41%, 10.34%, 5.56% respectively. Adenovirus was never identified in plants B and C as Rotavirus in plant C. The presence of faecal indicators was not predictive of the enteric virus presence, whereas a different circulation of Enteroviruses was found in the wastewater treatment plants. The study shows the importance and the usefulness of molecular methods to evaluate the virus circulation and the genetic variability of Enteroviruses.
2009
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/42 - IGIENE GENERALE E APPLICATA
English
Con Impact Factor ISI
biotechnology; genotyping; molecular biology; viruses; wastewater
Petrinca, A., Donia, D.t., Pierangeli, A., Gabrieli, R., Degener, A., Bonanni, E., et al. (2009). Presence and environmental circulation of enteric viruses in three different wastewater treatment plants. JOURNAL OF APPLIED MICROBIOLOGY, 106(5), 1608-1617 [10.1111/j.1365-2672.2008.04128.x].
Petrinca, A; Donia, Dt; Pierangeli, A; Gabrieli, R; Degener, A; Bonanni, E; Diaco, L; Cecchini, G; Anastasi, P; Divizia, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/38922
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