Exposure to environmental contaminants, like 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), leads to an increased susceptibility to infectious agents. Infection of bovine cells (MDBK) with Bovine Herpesvirus 1 (BHV-1) anticipates virus-induced apoptosis, suggesting an involvement of TCDD in virus infection. Herein we analyzed the effects of TCDD on apoptotic pathway in MDBK cells infected with BHV-1. After 12 h of infection, TCDD induced a significant increase in apoptotic cells. TCDD caused a dose-dependent up-regulation and anticipated activation of caspases 3, 8 and 9, with respect to unexposed groups. TCDD anticipated cleavage of PARP, compared to controls. Furthermore TCDD increased Bax and Bid levels, and decreased Bcl-2 and Bcl-XL levels. Such events took place earlier in exposed than unexposed cells. These results showed that TCDD influences BHV-1 induced apoptosis through members of Bcl-2 family and up-regulating activation of caspases.

Fiorito, R., Marfe, G., De Blasio, E., Granato, G., Tafani, M., De Martino, L., et al. (2008). 2,3,7,8-Tetrachlorodibenzo-p-dioxin regulates Bovine Herpesvirus type 1 induced apoptosis by modulating Bcl-2 family members. APOPTOSIS, 13(10), 1243-1252 [10.1007/s10495-008-0249-y].

2,3,7,8-Tetrachlorodibenzo-p-dioxin regulates Bovine Herpesvirus type 1 induced apoptosis by modulating Bcl-2 family members

FIORITO, ROBERTO;
2008-01-01

Abstract

Exposure to environmental contaminants, like 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), leads to an increased susceptibility to infectious agents. Infection of bovine cells (MDBK) with Bovine Herpesvirus 1 (BHV-1) anticipates virus-induced apoptosis, suggesting an involvement of TCDD in virus infection. Herein we analyzed the effects of TCDD on apoptotic pathway in MDBK cells infected with BHV-1. After 12 h of infection, TCDD induced a significant increase in apoptotic cells. TCDD caused a dose-dependent up-regulation and anticipated activation of caspases 3, 8 and 9, with respect to unexposed groups. TCDD anticipated cleavage of PARP, compared to controls. Furthermore TCDD increased Bax and Bid levels, and decreased Bcl-2 and Bcl-XL levels. Such events took place earlier in exposed than unexposed cells. These results showed that TCDD influences BHV-1 induced apoptosis through members of Bcl-2 family and up-regulating activation of caspases.
2008
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/18 - CHIRURGIA GENERALE
English
Con Impact Factor ISI
2,3,7,8 tetrachlorodibenzo para dioxin; caspase 3; caspase 8; caspase 9; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase; protein bcl 2; protein bcl xl; animal cell; apoptosis; article; cell count; controlled study; cow; disease predisposition; environmental exposure; enzyme activation; herpes; Infectious bovine rhinotracheitis virus; nonhuman; priority journal; upregulation; Animals; Apoptosis; Caspases; Cattle; Cell Count; Cell Line; Cell Membrane Permeability; Enzyme Activation; Herpesvirus 1, Bovine; Poly(ADP-ribose) Polymerases; Proto-Oncogene Proteins c-bcl-2; Tetrachlorodibenzodioxin; Bovinae; Bovine herpesvirus 1
Fiorito, R., Marfe, G., De Blasio, E., Granato, G., Tafani, M., De Martino, L., et al. (2008). 2,3,7,8-Tetrachlorodibenzo-p-dioxin regulates Bovine Herpesvirus type 1 induced apoptosis by modulating Bcl-2 family members. APOPTOSIS, 13(10), 1243-1252 [10.1007/s10495-008-0249-y].
Fiorito, R; Marfe, G; De Blasio, E; Granato, G; Tafani, M; De Martino, L; Montagnaro, S; Florio, S; Pagnini, U
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/38483
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