We have previously reported the isolation and characterization of a novel endothelial-restricted gene, Egfl7, that encodes a secreted protein of about 30-kDa. We and others demonstrated that Egfl7 is highly expressed by endothelial cells during embryonic development and becomes down-regulated in the adult vasculature. In the present paper, we show that during mouse embryonic development, Egfl7 is also expressed by primordial germ cells (PGC). Expression is down-regulated when PGCs differentiate into pro-spermatogonia and oogonia, and by 15.5 dpc Egfl7 can no longer be detected in the germ line of both sexes. Notably, Egfl7 is again transiently up-regulated in germ cells of the adult testis. In contrast, expression in the ovary remains limited to the vascular endothelium. Our results provide the first evidence of a non-endothelial expression of EGFL7 and suggest distinctive roles for Egfl7 in vascular development and germ cell differentiation. (C) 2008 Elsevier B.V. All rights reserved.

Campagnolo, L., Moscatelli, I., Pellegrini, M., Siracusa, G., Stuhlmann, H. (2008). Expression of EGFL7 in primordial germ cells and in adult ovaries and testes. GENE EXPRESSION PATTERNS, 8(6), 389-396 [10.1016/j.gep.2008.05.001].

Expression of EGFL7 in primordial germ cells and in adult ovaries and testes

CAMPAGNOLO, LUISA;SIRACUSA, GREGORIO;
2008-01-01

Abstract

We have previously reported the isolation and characterization of a novel endothelial-restricted gene, Egfl7, that encodes a secreted protein of about 30-kDa. We and others demonstrated that Egfl7 is highly expressed by endothelial cells during embryonic development and becomes down-regulated in the adult vasculature. In the present paper, we show that during mouse embryonic development, Egfl7 is also expressed by primordial germ cells (PGC). Expression is down-regulated when PGCs differentiate into pro-spermatogonia and oogonia, and by 15.5 dpc Egfl7 can no longer be detected in the germ line of both sexes. Notably, Egfl7 is again transiently up-regulated in germ cells of the adult testis. In contrast, expression in the ovary remains limited to the vascular endothelium. Our results provide the first evidence of a non-endothelial expression of EGFL7 and suggest distinctive roles for Egfl7 in vascular development and germ cell differentiation. (C) 2008 Elsevier B.V. All rights reserved.
2008
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/17 - ISTOLOGIA
English
Egf17 gene expression; Mouse embryonic development; Primordial germ cells; Spermatogenesis
Campagnolo, L., Moscatelli, I., Pellegrini, M., Siracusa, G., Stuhlmann, H. (2008). Expression of EGFL7 in primordial germ cells and in adult ovaries and testes. GENE EXPRESSION PATTERNS, 8(6), 389-396 [10.1016/j.gep.2008.05.001].
Campagnolo, L; Moscatelli, I; Pellegrini, M; Siracusa, G; Stuhlmann, H
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/38336
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