The interaction of the three main components of the mitochondrial membrane, namely cardiolipin, phosphatidylcholine, and phosphatidylethanolamine, has been studied investigating mixed cardiolipin-phosphatidylcholine and cardiolipin-phosphatidylethanolamine monolayers at different cardiolipin molar fractions. The thermodynamic behavior of the mixed monolayers was investigated by means of surface pressure and surface potential measurements, and atomic force microscopy was employed to characterize the morphology of the monolayers. Langmuir isotherms and surface potential curves show a regular behavior with a progressive transition toward the isotherm of the pure component. Positive deviations from ideality in the excess Gibbs energies of mixing suggest the presence of repulsive interactions in both systems. Analysis of partial molecular dipole moment indicates a discontinuity at a definite cardiolipin/phosphatidylethanolamine molar fraction, suggesting the formation of a stoichiometric complex; as a consequence, in mixed cardiolipin-phosphatidylethanolamine monolayers, a phase separation is observed at phosphatidylethanolamine excess. AFM measurements indicate the presence of two domains: one made by phosphatidylethanolamine and the other by a regular arrangement of phosphatidyl ethanol ami ne and cardiolipin at a fixed molecular ratio.

Sennato, S., Bordi, F., Cametti, C., Coluzza, C., Desideri, A., Rufini, S. (2005). Evidence of domain formation in cardiolipin-glycerophospholipid mixed monolayers. A thermodynamic and AFM study, 109(33), 15950-15957 [10.1021/jp051893q].

Evidence of domain formation in cardiolipin-glycerophospholipid mixed monolayers. A thermodynamic and AFM study

DESIDERI, ALESSANDRO;RUFINI, STEFANO
2005-01-01

Abstract

The interaction of the three main components of the mitochondrial membrane, namely cardiolipin, phosphatidylcholine, and phosphatidylethanolamine, has been studied investigating mixed cardiolipin-phosphatidylcholine and cardiolipin-phosphatidylethanolamine monolayers at different cardiolipin molar fractions. The thermodynamic behavior of the mixed monolayers was investigated by means of surface pressure and surface potential measurements, and atomic force microscopy was employed to characterize the morphology of the monolayers. Langmuir isotherms and surface potential curves show a regular behavior with a progressive transition toward the isotherm of the pure component. Positive deviations from ideality in the excess Gibbs energies of mixing suggest the presence of repulsive interactions in both systems. Analysis of partial molecular dipole moment indicates a discontinuity at a definite cardiolipin/phosphatidylethanolamine molar fraction, suggesting the formation of a stoichiometric complex; as a consequence, in mixed cardiolipin-phosphatidylethanolamine monolayers, a phase separation is observed at phosphatidylethanolamine excess. AFM measurements indicate the presence of two domains: one made by phosphatidylethanolamine and the other by a regular arrangement of phosphatidyl ethanol ami ne and cardiolipin at a fixed molecular ratio.
2005
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/11 - BIOLOGIA MOLECOLARE
English
Con Impact Factor ISI
Amines; Atomic force microscopy; Gibbs free energy; Isotherms; Phospholipids; Stoichiometry; Surface chemistry; Thermodynamics; Cardiolipin; Glycerophospholipids; Langmuir isotherms; Molar fraction; Monolayers; cardiolipin; dipalmitoylphosphatidylcholine; glycerophospholipid; phosphatidylethanolamine; article; atomic force microscopy; chemistry; surface property; surface tension; thermodynamics; 1,2-Dipalmitoylphosphatidylcholine; Cardiolipins; Glycerophospholipids; Microscopy, Atomic Force; Phosphatidylethanolamines; Surface Properties; Surface Tension; Thermodynamics
Sennato, S., Bordi, F., Cametti, C., Coluzza, C., Desideri, A., Rufini, S. (2005). Evidence of domain formation in cardiolipin-glycerophospholipid mixed monolayers. A thermodynamic and AFM study, 109(33), 15950-15957 [10.1021/jp051893q].
Sennato, S; Bordi, F; Cametti, C; Coluzza, C; Desideri, A; Rufini, S
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/37563
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