Amphiphilic poly(vinyl alcohol) randomly grafted with hydrophobic methacryloyl groups can form micelle-like particles by intra and interpolymeric association. Self-aggregation behaviour of the hydrophobically-modified polymer was investigated. The hydrophobized nanoparticles assist carbonic anhydrase B (CAB) refolding in a manner similar to the mechanism of molecular chaperones, namely by catching and releasing the protein. Irreversible CAB thermal denaturation is prevented by nanoparticle complexation and recovery of almost 100% of enzymatic activity is triggered by the ability of b-cyclodextrin to interact with the hydrophobic moieties. Structural and functional properties of micelle-like particles were discussed and interpreted in view of the stability and architecture of hydrophobic nanodomains.

Cavalieri, F., Chiessi, E., Paradossi, G. (2007). Chaperone-like activity of nanoparticles of hydrophobized poly(vinyl alcohol). SOFT MATTER, 3, 718-724 [10.1039/b618779j].

Chaperone-like activity of nanoparticles of hydrophobized poly(vinyl alcohol)

CAVALIERI, FRANCESCA;CHIESSI, ESTER;PARADOSSI, GAIO
2007-01-01

Abstract

Amphiphilic poly(vinyl alcohol) randomly grafted with hydrophobic methacryloyl groups can form micelle-like particles by intra and interpolymeric association. Self-aggregation behaviour of the hydrophobically-modified polymer was investigated. The hydrophobized nanoparticles assist carbonic anhydrase B (CAB) refolding in a manner similar to the mechanism of molecular chaperones, namely by catching and releasing the protein. Irreversible CAB thermal denaturation is prevented by nanoparticle complexation and recovery of almost 100% of enzymatic activity is triggered by the ability of b-cyclodextrin to interact with the hydrophobic moieties. Structural and functional properties of micelle-like particles were discussed and interpreted in view of the stability and architecture of hydrophobic nanodomains.
2007
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore CHIM/02 - CHIMICA FISICA
English
polymer micelle; poly(vinyl alcohol); nanoparticles; carbonic anhydrase B; CAB; Amphiphilic poly(vinyl alcohol)
Cavalieri, F., Chiessi, E., Paradossi, G. (2007). Chaperone-like activity of nanoparticles of hydrophobized poly(vinyl alcohol). SOFT MATTER, 3, 718-724 [10.1039/b618779j].
Cavalieri, F; Chiessi, E; Paradossi, G
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/35142
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 29
  • ???jsp.display-item.citation.isi??? 29
social impact