Fragile X mental retardation protein (FMRP), the protein responsible for the fragile X syndrome, is an RNA-binding protein involved in localization and translation of neuronal mRNAs. One of the RNAs known to interact with FMRP is the dendritic non-translatable brain cytoplasmic RNA 1 BC1 RNA that works as an adaptor molecule linking FMRP and some of its regulated mRNAs. Here, we showed that the N terminus of FMRP binds strongly and specifically to BC1 and to its potential human analog BC200. This region does not contain a motif known to specifically recognize RNA and thus constitutes a new RNA-binding motif. We further demonstrated that FMRP recognition involves the 5' stem loop of BC1 and that this is the region that exhibits complementarity to FMRP target mRNAs, raising the possibility that FMRP plays a direct role in BC1/ mRNA annealing.

Zalfa, F., Adinolfi, S., Napoli, I., Kuhn Holsken, E., Urlaub, H., Achsel, T., et al. (2005). Fragile X mental retardation protein (FMRP) binds specifically to the brain cytoplasmic RNAs BC1/BC200 via a novel RNA-binding motif. THE JOURNAL OF BIOLOGICAL CHEMISTRY, 280(39), 33403-33410 [10.1074/jbc.M504286200].

Fragile X mental retardation protein (FMRP) binds specifically to the brain cytoplasmic RNAs BC1/BC200 via a novel RNA-binding motif

BAGNI, CLAUDIA
2005-01-01

Abstract

Fragile X mental retardation protein (FMRP), the protein responsible for the fragile X syndrome, is an RNA-binding protein involved in localization and translation of neuronal mRNAs. One of the RNAs known to interact with FMRP is the dendritic non-translatable brain cytoplasmic RNA 1 BC1 RNA that works as an adaptor molecule linking FMRP and some of its regulated mRNAs. Here, we showed that the N terminus of FMRP binds strongly and specifically to BC1 and to its potential human analog BC200. This region does not contain a motif known to specifically recognize RNA and thus constitutes a new RNA-binding motif. We further demonstrated that FMRP recognition involves the 5' stem loop of BC1 and that this is the region that exhibits complementarity to FMRP target mRNAs, raising the possibility that FMRP plays a direct role in BC1/ mRNA annealing.
2005
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/13 - BIOLOGIA APPLICATA
English
Con Impact Factor ISI
Annealing; Cytology; Medical problems; Neurology; RNA; Brain cytoplasmic RNA; Fragile X mental retardation protein (FMRP); RNA-binding motif; Proteins; bovine serum albumin; fragile X mental retardation protein; messenger RNA; recombinant protein; RNA; RNA binding protein; amino terminal sequence; article; brain; carboxy terminal sequence; cross linking; cytoplasm; dissociation constant; electrophoretic mobility; fragile X syndrome; mass spectrometry; polyacrylamide gel electrophoresis; polymerase chain reaction; priority journal; protein binding; RNA binding; RNA translation; Base Sequence; Brain; Cross-Linking Reagents; Cytoplasm; Dendrites; Electrophoretic Mobility Shift Assay; Fragile X Syndrome; Gene Deletion; Humans; Models, Biological; Molecular Sequence Data; Neurons; Protein Structure, Tertiary; RNA, Messenger; RNA, Small Cytoplasmic; RNA-Binding Proteins; Ultraviolet Rays
Zalfa, F., Adinolfi, S., Napoli, I., Kuhn Holsken, E., Urlaub, H., Achsel, T., et al. (2005). Fragile X mental retardation protein (FMRP) binds specifically to the brain cytoplasmic RNAs BC1/BC200 via a novel RNA-binding motif. THE JOURNAL OF BIOLOGICAL CHEMISTRY, 280(39), 33403-33410 [10.1074/jbc.M504286200].
Zalfa, F; Adinolfi, S; Napoli, I; Kuhn Holsken, E; Urlaub, H; Achsel, T; Pastore, A; Bagni, C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/29174
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