The yellow-pigmented Gram-negative MCT13 strain was isolated from a drainage ditch within a disused system of constructed wetlands, flowing through uncultivated land. Growth on agar media was associated with clearing and pitting around the colonies, a feature never reported so far among sphingomonads. This trait, together with the unusual biochemical profile, let us to hypothesize that MCT13 belonged to a novel species. The 16S rDNA sequence shared the highest (98%) identity with uncultured sphingomonads. High Throughput DNA Sequencing confirmed the presence of putative agarase-like enzymes, and allowed to perform an Average Nucleotide Identity analysis, by comparing MCT13 with other Sphingomonas sp. deposited in the INSDC databases. The closest homolog (78.3%) is Sphingomonas koreensis NBRC_16723. As the species delineation cut-off is <95%, the hypothesis that MCT13 belongs to a novel species in the genus Sphingomonas is supported. The ability to degrade agar, together with the lack of known antibiotic resistances other than the genus-related resistance to streptomycin, make Sphingomonas sp. MCT13 a potentially interesting strain for the degradation of complex carbohydrates.

Marmo, P., D’Andrea, M.m., Casu, F., DI LALLO, G., Migliore, L., Thaller, M.c. (2017). MCT13: a newly discovered agarase-producing Sphingomonas sp. Isolated from freshwaters. In Abstract Book congresso.

MCT13: a newly discovered agarase-producing Sphingomonas sp. Isolated from freshwaters

MARMO P.;D’ANDREA M. M.;DI LALLO G.;MIGLIORE Luciana;THALLER Maria Cristina
2017-01-01

Abstract

The yellow-pigmented Gram-negative MCT13 strain was isolated from a drainage ditch within a disused system of constructed wetlands, flowing through uncultivated land. Growth on agar media was associated with clearing and pitting around the colonies, a feature never reported so far among sphingomonads. This trait, together with the unusual biochemical profile, let us to hypothesize that MCT13 belonged to a novel species. The 16S rDNA sequence shared the highest (98%) identity with uncultured sphingomonads. High Throughput DNA Sequencing confirmed the presence of putative agarase-like enzymes, and allowed to perform an Average Nucleotide Identity analysis, by comparing MCT13 with other Sphingomonas sp. deposited in the INSDC databases. The closest homolog (78.3%) is Sphingomonas koreensis NBRC_16723. As the species delineation cut-off is <95%, the hypothesis that MCT13 belongs to a novel species in the genus Sphingomonas is supported. The ability to degrade agar, together with the lack of known antibiotic resistances other than the genus-related resistance to streptomycin, make Sphingomonas sp. MCT13 a potentially interesting strain for the degradation of complex carbohydrates.
XXXII Congresso SIMGBM
Palermo
2017
XXXII
Rilevanza internazionale
2017
Settore BIO/19 - MICROBIOLOGIA GENERALE
English
Intervento a convegno
Marmo, P., D’Andrea, M.m., Casu, F., DI LALLO, G., Migliore, L., Thaller, M.c. (2017). MCT13: a newly discovered agarase-producing Sphingomonas sp. Isolated from freshwaters. In Abstract Book congresso.
Marmo, P; D’Andrea, Mm; Casu, F; DI LALLO, G; Migliore, L; Thaller, Mc
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/249723
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