The synergy of life sciences discoveries, biomolecular and protein engineering advances, and groundbreaking nanofabrication technologies, has introduced over the past years the wide use of the nanopore-based investigations of matter at the molecular level. This review focuses on the fundamental principles of alpha-hemolysin (alpha-HL) protein-based nanopores, as sensitive investigative tools that combine single-molecule detection with the ability to simultaneously manipulate single molecules, in otherwise complex samples. Herein, there are presented some of the efforts directed to control the capture dynamics and translocation speed of tailored polypeptides through the alpha-HL nanopore, by harnessing the electro-osmotic flow and nanopore-tweezing influence on individual molecules, which are engineered to resemble macrodipoles. The reported applications of this approach suggest a solution to enhance the temporal resolution of nanopore detection, prove the capability of the system in distinguishing between groups of distinct amino acids from the studied polypeptides, and propose a strategy to translate such single-molecule sensors in devices suitable for polypeptide sequencing at unimolecular level.

Asandei, A., Di Muccio, G., Schiopu, I., Mereuta, L., Dragomir, I.s., Chinappi, M., et al. (2020). Nanopore-Based Protein Sequencing Using Biopores: Current Achievements and Open Challenges. SMALL METHODS, 1900595 [10.1002/smtd.201900595].

Nanopore-Based Protein Sequencing Using Biopores: Current Achievements and Open Challenges

Chinappi M.;
2020-01-01

Abstract

The synergy of life sciences discoveries, biomolecular and protein engineering advances, and groundbreaking nanofabrication technologies, has introduced over the past years the wide use of the nanopore-based investigations of matter at the molecular level. This review focuses on the fundamental principles of alpha-hemolysin (alpha-HL) protein-based nanopores, as sensitive investigative tools that combine single-molecule detection with the ability to simultaneously manipulate single molecules, in otherwise complex samples. Herein, there are presented some of the efforts directed to control the capture dynamics and translocation speed of tailored polypeptides through the alpha-HL nanopore, by harnessing the electro-osmotic flow and nanopore-tweezing influence on individual molecules, which are engineered to resemble macrodipoles. The reported applications of this approach suggest a solution to enhance the temporal resolution of nanopore detection, prove the capability of the system in distinguishing between groups of distinct amino acids from the studied polypeptides, and propose a strategy to translate such single-molecule sensors in devices suitable for polypeptide sequencing at unimolecular level.
2020
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore ING-IND/06 - FLUIDODINAMICA
English
electrophysiology; molecular dynamics; polypeptide sequencing; protein nanopores; single-molecule sensing
Asandei, A., Di Muccio, G., Schiopu, I., Mereuta, L., Dragomir, I.s., Chinappi, M., et al. (2020). Nanopore-Based Protein Sequencing Using Biopores: Current Achievements and Open Challenges. SMALL METHODS, 1900595 [10.1002/smtd.201900595].
Asandei, A; Di Muccio, G; Schiopu, I; Mereuta, L; Dragomir, Is; Chinappi, M; Luchian, T
Articolo su rivista
File in questo prodotto:
File Dimensione Formato  
Asandei_et_al-2020-Small_Methods.pdf

solo utenti autorizzati

Tipologia: Versione Editoriale (PDF)
Licenza: Copyright dell'editore
Dimensione 1.51 MB
Formato Adobe PDF
1.51 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/247204
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 51
  • ???jsp.display-item.citation.isi??? 51
social impact