N-acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides (FAAs), a family of bioactive lipids including anandamide (AEA) as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin-layer chromatography (TLC).
Fezza, F., Mastrangelo, N., Maccarrone, M. (2016). Assay of NAPE-PLD Activity. In Mauro Maccarrone (a cura di), “Endocannabinoid Signaling: Methods and Protocols” (pp. 123-130). Springer [10.1007/978-1-4939-3539-0_13].
Assay of NAPE-PLD Activity
Fezza, Filomena;Mastrangelo, Nicolina;Maccarrone, Mauro
2016-01-01
Abstract
N-acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides (FAAs), a family of bioactive lipids including anandamide (AEA) as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin-layer chromatography (TLC).File in questo prodotto:
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