The fragile X mental retardation protein (FMRP) is lacking or mutated in patients with the fragile X syndrome (FXS), the most frequent form of inherited intellectual disability. FMRP affects metastasis formation in a mouse model for breast cancer. Here we show that FMRP is overexpressed in human melanoma with high Breslow thickness and high Clark level. Furthermore, metaanalysis of the TCGA melanoma data revealed that high levels of FMRP expression correlate significantly with metastatic tumor tissues, risk of relapsing and disease-free survival. Reduction of FMRP in metastatic melanoma cell lines impinges on cell migration, invasion and adhesion. Next-generation sequencing in human melanoma cells revealed that FMRP regulates a large number of mRNAs involved in relevant processes of melanoma progression. Our findings suggest an association between FMRP levels and the invasive phenotype in melanoma and might open new avenues towards the discovery of novel therapeutic targets.

Zalfa, F., Panasiti, V., Carotti, S., Zingariello, M., Perrone, G., Sancillo, L., et al. (2017). The fragile X mental retardation protein regulates tumor invasiveness-related pathways in melanoma cells. CELL DEATH & DISEASE, 8(11), e3169-e3169 [10.1038/cddis.2017.521].

The fragile X mental retardation protein regulates tumor invasiveness-related pathways in melanoma cells

De Luca A.;Farace M. G.
Membro del Collaboration Group
;
Bagni C.
Conceptualization
2017-01-01

Abstract

The fragile X mental retardation protein (FMRP) is lacking or mutated in patients with the fragile X syndrome (FXS), the most frequent form of inherited intellectual disability. FMRP affects metastasis formation in a mouse model for breast cancer. Here we show that FMRP is overexpressed in human melanoma with high Breslow thickness and high Clark level. Furthermore, metaanalysis of the TCGA melanoma data revealed that high levels of FMRP expression correlate significantly with metastatic tumor tissues, risk of relapsing and disease-free survival. Reduction of FMRP in metastatic melanoma cell lines impinges on cell migration, invasion and adhesion. Next-generation sequencing in human melanoma cells revealed that FMRP regulates a large number of mRNAs involved in relevant processes of melanoma progression. Our findings suggest an association between FMRP levels and the invasive phenotype in melanoma and might open new avenues towards the discovery of novel therapeutic targets.
2017
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/13 - BIOLOGIA APPLICATA
English
Fragile X Mental Retardation Protein; Humans; Melanoma; Neoplasm Invasiveness; Transfection
Zalfa, F., Panasiti, V., Carotti, S., Zingariello, M., Perrone, G., Sancillo, L., et al. (2017). The fragile X mental retardation protein regulates tumor invasiveness-related pathways in melanoma cells. CELL DEATH & DISEASE, 8(11), e3169-e3169 [10.1038/cddis.2017.521].
Zalfa, F; Panasiti, V; Carotti, S; Zingariello, M; Perrone, G; Sancillo, L; Pacini, L; Luciani, F; Roberti, V; D'Amico, S; Coppola, R; Abate, So; Rana, Ra; De Luca, A; Fiers, M; Melocchi, V; Bianchi, F; Farace, Mg; Achsel, T; Marine, J-; Morini, S; Bagni, C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/244627
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