Association between extremely low frequency electromagnetic fields (ELF-EMF) and human cancers is controversial, and few studies have been conducted on their influence on oncogenic viruses. We studied the effects of 1 mT, 50 Hz sine waves, applied for 24-72 h, on Kaposi's sarcoma (KS)-associated herpesvirus (KSHV or HHV-8) in BCBL-1, a latently infected primary effusion lymphoma (PEL) cell line. ELF-EMF exposure did not affect the growth and viability of BCBL-1 cells, either stimulated or not with TPA. The total amount of KSHV DNA detected in ELF-EMF exposed cultures not stimulated with TPA did not differ from that of the unexposed controls (P = ns). However, in the presence of TPA stimulation, total KSHV DNA content was found higher in ELF-EMF exposed than in control BCBL-1 cultures (P = .024) at 72 h exposure, but not earlier. Viral DNA increase significantly correlated with increased mean fluorescence intensity/cell for the lytic antigen gp K8.1A/B (P < .01), but not with percentage of gp K8.1A/B-positive cells or of cells containing virions. Viral progeny produced under ELF-EMF exposure consisted mainly of defective viral particles.

Pica, F., Serafino, A., Divizia, M., Donia, D.t., Fraschetti, M., Sinibaldi Salimei, P., et al. (2006). Effect of extremely low frequency electromagnetic fields (ELF-EMF) on Kaposi's sarcoma-associated herpes virus in BCBL-1 cells. BIOELECTROMAGNETICS, 27(3), 226-232 [10.1002/bem.20198].

Effect of extremely low frequency electromagnetic fields (ELF-EMF) on Kaposi's sarcoma-associated herpes virus in BCBL-1 cells

PICA, FRANCESCA;DIVIZIA, MAURIZIO;DONIA, DOMENICA TOMMASA;Sinibaldi Salimei, P;GIGANTI, MARIA GABRIELLA;
2006-04-01

Abstract

Association between extremely low frequency electromagnetic fields (ELF-EMF) and human cancers is controversial, and few studies have been conducted on their influence on oncogenic viruses. We studied the effects of 1 mT, 50 Hz sine waves, applied for 24-72 h, on Kaposi's sarcoma (KS)-associated herpesvirus (KSHV or HHV-8) in BCBL-1, a latently infected primary effusion lymphoma (PEL) cell line. ELF-EMF exposure did not affect the growth and viability of BCBL-1 cells, either stimulated or not with TPA. The total amount of KSHV DNA detected in ELF-EMF exposed cultures not stimulated with TPA did not differ from that of the unexposed controls (P = ns). However, in the presence of TPA stimulation, total KSHV DNA content was found higher in ELF-EMF exposed than in control BCBL-1 cultures (P = .024) at 72 h exposure, but not earlier. Viral DNA increase significantly correlated with increased mean fluorescence intensity/cell for the lytic antigen gp K8.1A/B (P < .01), but not with percentage of gp K8.1A/B-positive cells or of cells containing virions. Viral progeny produced under ELF-EMF exposure consisted mainly of defective viral particles.
apr-2006
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/42 - IGIENE GENERALE E APPLICATA
English
Con Impact Factor ISI
Cell Line, Tumor; Electromagnetic Fields; Cell Survival; Lymphoma; DNA, Viral; Radiation Dosage; Dose-Response Relationship, Radiation; Virus Replication; Humans; Herpesvirus 8, Human; Electricity
Pica, F., Serafino, A., Divizia, M., Donia, D.t., Fraschetti, M., Sinibaldi Salimei, P., et al. (2006). Effect of extremely low frequency electromagnetic fields (ELF-EMF) on Kaposi's sarcoma-associated herpes virus in BCBL-1 cells. BIOELECTROMAGNETICS, 27(3), 226-232 [10.1002/bem.20198].
Pica, F; Serafino, A; Divizia, M; Donia, Dt; Fraschetti, M; Sinibaldi Salimei, P; Giganti, Mg; Volpi, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/15945
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