Melanoma is a widespread cancer with poor prognosis. Female hormones are known to be capable of influencing melanoma progression but clinical data related to pregnancy, oral contraception and hormone replacement therapy are controversial. A few reports show that in vitro progesterone (PG) affects melanoma growth in nuclear progesterone receptor (nPR)-positive and nPR-negative cells, but the experimental protocols used are quite different and the results are not univocal. Further research on this topic is thus needed especially in view of the widespread use of PG in clinical practice. In this study, we used human melanoma cells (A-375), which were cultured in vitro in the presence or absence of a wide range of PG concentrations (from 0.01 to 1000 M) in single treatment. Daily cell count, cell cycle analysis and apoptosis assay were performed. Our results show that the low PG concentrations (from 0.01 to 1.0 M) promote a significant increase of melanoma cell proliferation but this growth-stimulatory effect is not observed at 10 M PG and the higher PG concentrations (i.e. 100 and 1000 M) induce a cell density reduction which is the result of both cell cycle arrest and apoptosis. These findings confirm and extend previous observations reported in the international literature. A higher caution in the clinical use of progesterone is thus mandatory, since PG concentrations capable of stimulating melanoma cell proliferation are very close to those commonly used in a wide spectrum of physio-pathological conditions.

Moroni, G., Gaziano, R., Bue', M.c., Agostini, M., Perno, C.f., SINIBALDI VALLEBONA, P., et al. (2015). Progesterone and Melanoma Cells: An Old Story Suspended between Life and Death. JOURNAL OF STEROIDS & HORMONAL SCIENCE, 06(03) [10.4172/2157-7536.1000158].

Progesterone and Melanoma Cells: An Old Story Suspended between Life and Death

GAZIANO, ROBERTA;BUE', MARIA CRISTINA;AGOSTINI, MASSIMILIANO;PERNO, CARLO FEDERICO;SINIBALDI VALLEBONA, PAOLA;PICA, FRANCESCA
2015-08-24

Abstract

Melanoma is a widespread cancer with poor prognosis. Female hormones are known to be capable of influencing melanoma progression but clinical data related to pregnancy, oral contraception and hormone replacement therapy are controversial. A few reports show that in vitro progesterone (PG) affects melanoma growth in nuclear progesterone receptor (nPR)-positive and nPR-negative cells, but the experimental protocols used are quite different and the results are not univocal. Further research on this topic is thus needed especially in view of the widespread use of PG in clinical practice. In this study, we used human melanoma cells (A-375), which were cultured in vitro in the presence or absence of a wide range of PG concentrations (from 0.01 to 1000 M) in single treatment. Daily cell count, cell cycle analysis and apoptosis assay were performed. Our results show that the low PG concentrations (from 0.01 to 1.0 M) promote a significant increase of melanoma cell proliferation but this growth-stimulatory effect is not observed at 10 M PG and the higher PG concentrations (i.e. 100 and 1000 M) induce a cell density reduction which is the result of both cell cycle arrest and apoptosis. These findings confirm and extend previous observations reported in the international literature. A higher caution in the clinical use of progesterone is thus mandatory, since PG concentrations capable of stimulating melanoma cell proliferation are very close to those commonly used in a wide spectrum of physio-pathological conditions.
24-ago-2015
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
English
Con Impact Factor ISI
Progesterone; Cancer; Human melanoma; Steroid hormones; Cell proliferation; Cell-cycle; Apoptosis
Moroni, G., Gaziano, R., Bue', M.c., Agostini, M., Perno, C.f., SINIBALDI VALLEBONA, P., et al. (2015). Progesterone and Melanoma Cells: An Old Story Suspended between Life and Death. JOURNAL OF STEROIDS & HORMONAL SCIENCE, 06(03) [10.4172/2157-7536.1000158].
Moroni, G; Gaziano, R; Bue', Mc; Agostini, M; Perno, Cf; SINIBALDI VALLEBONA, P; Pica, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/158017
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