Stabilization and electrical contacting of redox enzymes with electrodes are fundamental requirements for bioelectronics devices, including biosensors and enzyme fuel cells (EFCs). In this study, we show increased glucose oxidase (GOx) stability by immobilization with Nafion. The immobilization process affected GOx conformation but was not detrimental to its activity, which was maintained for more than 120 days. The GOx/Nafion system was interfaced to a carbon cloth electrode and assembled in a prototypal EFC fed with glucose. Polarization and power density curves demonstrated that GOx/Nafion system was able to generate power, exploiting a Nafion-assisted electron transfer process to the electrode. Our findings are consistent with the onset of pH-dependent conformational equilibrium for the enzyme secondary structure and its active site. Significantly, the protective effect exerted by Nafion on the enzyme structure may be tuned by varying parameters such as the pH to fabricate durable EFCs with good electrocatalytic performance.

Mecheri, B., De Porcellinis, D., Campana, P., Rainer, A., Trombetta, M., Marletta, A., et al. (2015). Tuning Structural Changes in Glucose Oxidase for Enzyme Fuel Cell Applications. ACS APPLIED MATERIALS & INTERFACES, 7(51), 28311-28318 [10.1021/acsami.5b08610].

Tuning Structural Changes in Glucose Oxidase for Enzyme Fuel Cell Applications

MECHERI, BARBARA;RAINER, ALBERTO;LICOCCIA, SILVIA
2015-01-01

Abstract

Stabilization and electrical contacting of redox enzymes with electrodes are fundamental requirements for bioelectronics devices, including biosensors and enzyme fuel cells (EFCs). In this study, we show increased glucose oxidase (GOx) stability by immobilization with Nafion. The immobilization process affected GOx conformation but was not detrimental to its activity, which was maintained for more than 120 days. The GOx/Nafion system was interfaced to a carbon cloth electrode and assembled in a prototypal EFC fed with glucose. Polarization and power density curves demonstrated that GOx/Nafion system was able to generate power, exploiting a Nafion-assisted electron transfer process to the electrode. Our findings are consistent with the onset of pH-dependent conformational equilibrium for the enzyme secondary structure and its active site. Significantly, the protective effect exerted by Nafion on the enzyme structure may be tuned by varying parameters such as the pH to fabricate durable EFCs with good electrocatalytic performance.
2015
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/07 - FONDAMENTI CHIMICI DELLE TECNOLOGIE
English
Nafion; enzymatic fuel cells; enzyme activity; enzyme secondary structure; glucose oxidase
http://pubs.acs.org/doi/abs/10.1021/acsami.5b08610
Mecheri, B., De Porcellinis, D., Campana, P., Rainer, A., Trombetta, M., Marletta, A., et al. (2015). Tuning Structural Changes in Glucose Oxidase for Enzyme Fuel Cell Applications. ACS APPLIED MATERIALS & INTERFACES, 7(51), 28311-28318 [10.1021/acsami.5b08610].
Mecheri, B; De Porcellinis, D; Campana, P; Rainer, A; Trombetta, M; Marletta, A; Oliveira, O; Licoccia, S
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/149607
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