BACKGROUND: Thymosin α-1 (Tα1) exploits a specific action on lymphoid cells and is able to induce in peripheral blood mononuclear cells (PBMCs) a strong transcriptional response. CD8 antiviral factor activity plays a role in the control or prevention of HIV-1 infection by a non-cytolytic mechanism. The ability of Tα1 to modulate the release of antiretroviral soluble factors by CD8(+) cells was investigated. METHODS: Supernatants from lipopolysaccharide (LPS) stimulated CD8(+)-isolated cells treated with Tα1 were screened on in vitro infection of human monocyte-derived macrophages (MDMs) and PBMCs with HIV-1, and of PBMCs with human T lymphotropic virus 1 (HTLV-1). In CD8(+) cells, as well as in PBMCs of healthy donors as from HIV(+) individuals, a microarray analysis to assess the transcriptional response after treatment was performed. RESULTS: Tα1 potentiates the release, in LPS-stimulated CD8(+) cells, of soluble factors able to inhibit both in vitro HIV-1 infection of MDMs and PBMCs and in vitro HTLV-1 infection of PBMCs. A distinctive transcriptional profile was induced by Tα1 in PBMCs from HIV(+) donors. CONCLUSIONS: These findings suggest that Tα1 would represent a re-evaluated approach to antiretroviral therapy in combination with innovative treatments and with vaccine administration.

Matteucci, C., Minutolo, A., Pollicita, M., Balestrieri, E., Grelli, S., D'Ettorre, G., et al. (2015). Thymosin α 1 potentiates the release by CD8(+) cells of soluble factors able to inhibit HIV-1 and human T lymphotropic virus 1 infection in vitro. EXPERT OPINION ON BIOLOGICAL THERAPY, 15 Suppl 1(sup1), 83-100 [10.1517/14712598.2015.1021677].

Thymosin α 1 potentiates the release by CD8(+) cells of soluble factors able to inhibit HIV-1 and human T lymphotropic virus 1 infection in vitro

MATTEUCCI, CLAUDIA
;
MINUTOLO, ANTONELLA;POLLICITA, MICHELA;BALESTRIERI, EMANUELA;GRELLI, SANDRO;AQUARO, STEFANO;SINIBALDI VALLEBONA, PAOLA;MACCHI, BEATRICE;PERNO, CARLO FEDERICO;MASTINO, ANTONIO;GARACI, ENRICO
2015-06-01

Abstract

BACKGROUND: Thymosin α-1 (Tα1) exploits a specific action on lymphoid cells and is able to induce in peripheral blood mononuclear cells (PBMCs) a strong transcriptional response. CD8 antiviral factor activity plays a role in the control or prevention of HIV-1 infection by a non-cytolytic mechanism. The ability of Tα1 to modulate the release of antiretroviral soluble factors by CD8(+) cells was investigated. METHODS: Supernatants from lipopolysaccharide (LPS) stimulated CD8(+)-isolated cells treated with Tα1 were screened on in vitro infection of human monocyte-derived macrophages (MDMs) and PBMCs with HIV-1, and of PBMCs with human T lymphotropic virus 1 (HTLV-1). In CD8(+) cells, as well as in PBMCs of healthy donors as from HIV(+) individuals, a microarray analysis to assess the transcriptional response after treatment was performed. RESULTS: Tα1 potentiates the release, in LPS-stimulated CD8(+) cells, of soluble factors able to inhibit both in vitro HIV-1 infection of MDMs and PBMCs and in vitro HTLV-1 infection of PBMCs. A distinctive transcriptional profile was induced by Tα1 in PBMCs from HIV(+) donors. CONCLUSIONS: These findings suggest that Tα1 would represent a re-evaluated approach to antiretroviral therapy in combination with innovative treatments and with vaccine administration.
giu-2015
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
English
Con Impact Factor ISI
CD8 antiviral factor; HIV-1; Thymosin α 1; chemokines; human T lymphotropic virus 1; immunotherapy
http://www.tandfonline.com/doi/abs/10.1517/14712598.2015.1021677?journalCode=iebt20
Matteucci, C., Minutolo, A., Pollicita, M., Balestrieri, E., Grelli, S., D'Ettorre, G., et al. (2015). Thymosin α 1 potentiates the release by CD8(+) cells of soluble factors able to inhibit HIV-1 and human T lymphotropic virus 1 infection in vitro. EXPERT OPINION ON BIOLOGICAL THERAPY, 15 Suppl 1(sup1), 83-100 [10.1517/14712598.2015.1021677].
Matteucci, C; Minutolo, A; Pollicita, M; Balestrieri, E; Grelli, S; D'Ettorre, G; Vullo, V; Bucci, I; Luchini, A; Aquaro, S; SINIBALDI VALLEBONA, P; Macchi, B; Perno, Cf; Mastino, A; Garaci, E
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/129831
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