The KDEL retention signal, when added at the C-terminal of the constant region of light and heavy chains of immunoglobulins is able to efficiently retain assembled immunoglobulins only in cells of nonlymphoid origin. In transfected myeloma cells the wild type and the KDEL-Ig mutants are secreted with the same efficiency. This phenomenon is not due to a proteolytic cleavage of the KDEL signal nor to a lack of intermolecular disulfide bond formation and is not due to an impaired recognition of the KDEL signal in myeloma cells. Thus, the constitutive secretion of assembled immunoglobulins, currently considered to follow a default process, appears to be regulated by a mechanism that is able to overcome an efficient ER retention system.

Biocca, S., Tafani, M., Cattaneo, A. (1998). Assembled IgG molecules are exported from the endoplasmic reticulum in myeloma cells despite the retention signal sekdel. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 246(2), 518-523 [10.1006/bbrc.1998.8572].

Assembled IgG molecules are exported from the endoplasmic reticulum in myeloma cells despite the retention signal sekdel

BIOCCA, SILVIA;
1998-01-01

Abstract

The KDEL retention signal, when added at the C-terminal of the constant region of light and heavy chains of immunoglobulins is able to efficiently retain assembled immunoglobulins only in cells of nonlymphoid origin. In transfected myeloma cells the wild type and the KDEL-Ig mutants are secreted with the same efficiency. This phenomenon is not due to a proteolytic cleavage of the KDEL signal nor to a lack of intermolecular disulfide bond formation and is not due to an impaired recognition of the KDEL signal in myeloma cells. Thus, the constitutive secretion of assembled immunoglobulins, currently considered to follow a default process, appears to be regulated by a mechanism that is able to overcome an efficient ER retention system.
1998
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA
English
Con Impact Factor ISI
Amino Acid Sequence; Animals; Base Sequence; Biological Transport, Active; COS Cells; Cell Line; DNA Primers; Endoplasmic Reticulum; Immunoglobulin G; Mice; Models, Biological; Multiple Myeloma; Protein Sorting Signals; Rats; Transfection; Tumor Cells, Cultured
Biocca, S., Tafani, M., Cattaneo, A. (1998). Assembled IgG molecules are exported from the endoplasmic reticulum in myeloma cells despite the retention signal sekdel. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 246(2), 518-523 [10.1006/bbrc.1998.8572].
Biocca, S; Tafani, M; Cattaneo, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/127601
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