MRS6 is a newly-identified gene in the yeast Saccharomyces cerevisiae. Its product Mrs6p shows significant homology to the mammalian GDP dissociation inhibitor (GDI) of Rab/Ypt-type small G proteins and to the human choroideraemia protein (CHM), the component A of Rab-specific GGTase II. The interaction of Mrs6p with G proteins is indicated by our observation that the MRS6 gene suppresses the effect of a temperature-sensitive ypt1 mutation. Disruption of the MRS6 gene is lethal to haploid yeast cells. This is consistent, with the notion that Mrs6p is interacting with Rab/Ypt- type small G proteins, which are known to have essential functions in vesicular transport. Unexpectedly, the MRS6 gene product also affects mitochondrial functions as revealed by the facts that high-copy numbers of MRS6 (1) suppress the pet(-) phenotype of mrs2-1 mutant strains and (2) cause a weak pet(-) phenotype in wild-type strains. We conclude from these results that the MRS6 gene product has a vital function in connection with Rab/Ypt-type proteins in the cytoplasm and, in addition, affects mitochondrial functions.

Ragnini, A., Teply, R., Waldherr, M., Voskova, A., Schweyen, R. (1994). The yeast protein mrs6p, a homologue of the RabGDI and human choroideraemia proteins, affects cytoplasmic and mitochondrial functions. CURRENT GENETICS, 26(4), 308-314 [10.1007/BF00310494].

The yeast protein mrs6p, a homologue of the RabGDI and human choroideraemia proteins, affects cytoplasmic and mitochondrial functions

RAGNINI, ANTONELLA;
1994-01-01

Abstract

MRS6 is a newly-identified gene in the yeast Saccharomyces cerevisiae. Its product Mrs6p shows significant homology to the mammalian GDP dissociation inhibitor (GDI) of Rab/Ypt-type small G proteins and to the human choroideraemia protein (CHM), the component A of Rab-specific GGTase II. The interaction of Mrs6p with G proteins is indicated by our observation that the MRS6 gene suppresses the effect of a temperature-sensitive ypt1 mutation. Disruption of the MRS6 gene is lethal to haploid yeast cells. This is consistent, with the notion that Mrs6p is interacting with Rab/Ypt- type small G proteins, which are known to have essential functions in vesicular transport. Unexpectedly, the MRS6 gene product also affects mitochondrial functions as revealed by the facts that high-copy numbers of MRS6 (1) suppress the pet(-) phenotype of mrs2-1 mutant strains and (2) cause a weak pet(-) phenotype in wild-type strains. We conclude from these results that the MRS6 gene product has a vital function in connection with Rab/Ypt-type proteins in the cytoplasm and, in addition, affects mitochondrial functions.
1994
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
Choroideraemia, Rab escort protein, protein trafficking, genomic, yeast
Ragnini, A., Teply, R., Waldherr, M., Voskova, A., Schweyen, R. (1994). The yeast protein mrs6p, a homologue of the RabGDI and human choroideraemia proteins, affects cytoplasmic and mitochondrial functions. CURRENT GENETICS, 26(4), 308-314 [10.1007/BF00310494].
Ragnini, A; Teply, R; Waldherr, M; Voskova, A; Schweyen, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/90972
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