Effect of salt stress was examined in in vitro shoot cultures of Myrtus communis L. a species of the Mediterranean maquis. To determine the effects of high salt concentrations on myrtle plantlets and contribute toward understanding the mechanisms adopted from this species to counteract soil salinity, in vitro rooted shoots were transferred to a liquid culture medium containing 0, 125 or 250 mM NaCl for 30 days. After 15 and 30 days of in vitro culture, shoot and root growth, chlorosis and necrosis extension, chlorophylls, carotenoids, proline, arginine, cysteine and total sugars content, as well as guaiacol peroxidase (G-POD, EC 1.11.1.7) and ascorbate peroxidase (APX, EC 1.11.1.11) activities were determined. In treated plants shoot and root growth, as well as chlorophyll content, significantly decreased, while carotenoids content was not affected by the NaCl treatment. Among osmolytes, proline did not significantly increase, arginine and cysteine decreased, while total sugars were found to be higher in the treated plants than in the control. Enhancement of G-POD and APX activities was positively related to increasing salt concentrations in the culture media, regardless of the exposure time. Salt-treated plants did not show significant changes in lipid peroxidation or DNA fragmentation after 30 days salt treatment, regardless of the NaCl concentrations applied. The results represent a contribution towards understanding the mechanisms adopted by this species to high salinity

Di Cori, P., Lucioli, S., Frattarelli, A., Nota, P., Tel Or, E., Benyamini, E., et al. (2013). Characterization of the response of in vitro cultured Myrtus communis L. plants to high concentrations of NaCl. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 73, 420-426 [http://dx. DOI,org/10.1016/j.plaphy.2013.10.026].

Characterization of the response of in vitro cultured Myrtus communis L. plants to high concentrations of NaCl.

FORNI, CINZIA
2013-01-01

Abstract

Effect of salt stress was examined in in vitro shoot cultures of Myrtus communis L. a species of the Mediterranean maquis. To determine the effects of high salt concentrations on myrtle plantlets and contribute toward understanding the mechanisms adopted from this species to counteract soil salinity, in vitro rooted shoots were transferred to a liquid culture medium containing 0, 125 or 250 mM NaCl for 30 days. After 15 and 30 days of in vitro culture, shoot and root growth, chlorosis and necrosis extension, chlorophylls, carotenoids, proline, arginine, cysteine and total sugars content, as well as guaiacol peroxidase (G-POD, EC 1.11.1.7) and ascorbate peroxidase (APX, EC 1.11.1.11) activities were determined. In treated plants shoot and root growth, as well as chlorophyll content, significantly decreased, while carotenoids content was not affected by the NaCl treatment. Among osmolytes, proline did not significantly increase, arginine and cysteine decreased, while total sugars were found to be higher in the treated plants than in the control. Enhancement of G-POD and APX activities was positively related to increasing salt concentrations in the culture media, regardless of the exposure time. Salt-treated plants did not show significant changes in lipid peroxidation or DNA fragmentation after 30 days salt treatment, regardless of the NaCl concentrations applied. The results represent a contribution towards understanding the mechanisms adopted by this species to high salinity
2013
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/01 - BOTANICA GENERALE
English
Con Impact Factor ISI
Enzymatic and non-enzymatic antioxidants In vitro culture Myrtle Salt stress
Di Cori, P., Lucioli, S., Frattarelli, A., Nota, P., Tel Or, E., Benyamini, E., et al. (2013). Characterization of the response of in vitro cultured Myrtus communis L. plants to high concentrations of NaCl. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 73, 420-426 [http://dx. DOI,org/10.1016/j.plaphy.2013.10.026].
Di Cori, P; Lucioli, S; Frattarelli, A; Nota, P; Tel Or, E; Benyamini, E; Gottlieb, H; Caboni, E; Forni, C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/89229
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